Forskolin stimulates the conversion of tyrosine to dopamine in catecholaminergic neural tissue

Forskolin stimulates the conversion of tyrosine to dopamine in slices and synaptosomes from rat striatum, in synaptosomes from rat hypothalamus, and in slices from bovine retina. In striatal synaptosomes, there is an approximately 80% stimulation of dopamine formation from tyrosine, but no effect wi...

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Bibliographic Details
Published inBrain research Vol. 264; no. 1; p. 173
Main Authors Katz, I R, Smith, D, Makman, M H
Format Journal Article
LanguageEnglish
Published Netherlands 28.03.1983
Subjects
Animals
Bucladesine - pharmacology
Catecholamines - metabolism
Cattle
Colforsin
Corpus Striatum - drug effects
Corpus Striatum - metabolism
Dihydroxyphenylalanine - metabolism
Diterpenes - pharmacology
Dopamine - biosynthesis
Female
Hypothalamus - drug effects
Hypothalamus - metabolism
In Vitro Techniques
Kinetics
Rats
Retina - drug effects
Retina - metabolism
Synaptosomes - drug effects
Synaptosomes - metabolism
Tyrosine - metabolism
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Summary:Forskolin stimulates the conversion of tyrosine to dopamine in slices and synaptosomes from rat striatum, in synaptosomes from rat hypothalamus, and in slices from bovine retina. In striatal synaptosomes, there is an approximately 80% stimulation of dopamine formation from tyrosine, but no effect with DOPA as substrate, suggesting that the effect is on tyrosine hydroxylase. Stimulation is saturable with a half-maximal effect at under 1 microM forskolin. Forskolin stimulation is additive with, and hence independent of , activation due to KCl, but not that due to dibutyryl-cyclic AMP. Stimulation of dopamine synthesis by forskolin may provide an approach for elucidating the regulation of the adenylate cyclase system associated with catecholaminergic nerve endings.
ISSN:0006-8993
DOI:10.1016/0006-8993(83)91139-3