Label-free hyperspectral nonlinear optical microscopy of the biofuel micro-algae Haematococcus Pluvialis

We consider multi-modal four-wave mixing microscopies to be ideal tools for the in vivo study of carotenoid distributions within the important biofuel microalgae Haematococcus pluvialis. We show that hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy generates non-invasive, quanti...

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Bibliographic Details
Published inBiomedical optics express Vol. 5; no. 10; pp. 3391 - 3402
Main Authors Barlow, Aaron M, Slepkov, Aaron D, Ridsdale, Andrew, McGinn, Patrick J, Stolow, Albert
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 01.10.2014
Subjects
(170.1420) Biology
(170.1530) Cell analysis
(300.6230) Spectroscopy, coherent anti-Stokes Raman scattering
(300.6290) Spectroscopy, four-wave mixing
(180.4315) Nonlinear microscopy
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Summary:We consider multi-modal four-wave mixing microscopies to be ideal tools for the in vivo study of carotenoid distributions within the important biofuel microalgae Haematococcus pluvialis. We show that hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy generates non-invasive, quantitative real-time concentrations maps of intracellular carotenoid distributions in live algae.
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ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.5.003391