Selective determination of trace 17β-estradiol in dairy and meat samples by molecularly imprinted solid-phase extraction and HPLC

• Published in: Food chemistry Vol. 126; no. 4; pp. 1916 - 1925
• Main Authors: Shi, Yun, Peng, Dong-Dong, Shi, Chang-Hua, Zhang, Xia, Xie, Ya-Ting, Lu, Bin
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.06.2011; [Amsterdam]: Elsevier Science; Elsevier
• Subjects: 17 beta -Estradiol; accuracy; Biological and medical sciences; Dairy and meat samples; dairy products; detection; drug residues; equipment performance; estradiol; food analysis; food composition; food contamination; Food industries; food quality; Fundamental and applied biological sciences. Psychology; high performance liquid chromatography; HPLC-UV; meat; Meat and meat product industries; meat composition; MISPE; Modified precipitation polymerisation; molecular conformation; Molecularly imprinted microspheres; molecularly imprinted solid-phase extraction; selective determination; solid phase extraction; Trace 17β-estradiol; veterinary drugs; Trace 17β-estradiol; Dairy and meat samples; Modified precipitation polymerisation; MISPE; Molecularly imprinted microspheres; HPLC-UV; Solid phase extraction; Microsphere; Sample; Meat product; HPLC chromatography; Estradiol; Meat; Trace; Precipitation; Sex steroid hormone
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2010.12.020

► Off-line MISPE and HPLC-UV detected trace E2 in dairy and meat samples accurately. ► The high clean-up efficiency of MISPE increased sensitivity and accuracy of HPLC. ► MISPE have better HPLC separation and higher recoveries than C18 SPE. ► MISPE and HPLC-UV can be used for routine analysis of E2 in food samples. Molecularly imprinted solid-phase extraction (MISPE) combined with HPLC were used to detect trace 17β-estradiol (E2) in different dairy and meat samples. E2 imprinted mono-dispersed microspheres prepared by modified precipitation polymerisation method were used as MISPE sorbents. Under optimum MISPE and HPLC conditions, trace (0.116–0.461nmolkg−1) E2 in different dairy and meat samples can be detected accurately using UV detector when large amount of sample (500g) was analysed. Recoveries of E2 from spiked (0.1–5nmolkg−1) milk, yogurt, beef, pork and chicken were higher than 74.18%, 71.92%, 67.21%, 64.20% and 65.93%, respectively with RSDs less than 8.38%. MISPE can enrich E2 selectively, which is helpful to have better HPLC separation and higher recoveries than C18 SPE. MISPE combined with HPLC-UV are reliable methods to determine trace E2 in dairy and meat samples with high accuracy and repeatability.