Changes of reliability and efficiency of micronucleus bioassay in Vicia faba after exposure to metal contamination for several generations
Mitotic root micronucleus (MCN) frequency in Vicia faba as a bioassay, is primarily based on the extent of the sentinel Vicia response in terms of cytogenetic damage quantitatively or qualitatively to indicate the presence of mutagenic contaminants. This paper describes an investigation designed to...
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Published in | Environmental and experimental botany Vol. 44; no. 1; pp. 83 - 92 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
01.08.2000
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | Mitotic root micronucleus (MCN) frequency in
Vicia faba as a bioassay, is primarily based on the extent of the sentinel
Vicia response in terms of cytogenetic damage quantitatively or qualitatively to indicate the presence of mutagenic contaminants. This paper describes an investigation designed to assess changes in MCN frequencies of
Vicia faba from three generation plants obtained, respectively from a reference site (RS) and a metal-contaminated experimental field (EF) in the bioassay of mutagenic Cd
2+ and NaN
3. The background value, dose–response to Cd
2+ and to NaN
3 in three generation (F
1, F
2 and F
3) plants of the EF and the initial (F
0) plants were determined in terms of MCN frequencies. With more generations of growing
Vicia plants in the EF, a higher background value of MCN frequency, a lower slope value in the regression equation, a smaller ratio of MCN frequency between the control and treatment in the same generation and larger perturbation values were observed. This denotes that the decreased reliability and efficiency are represented in
Vicia plants from the EF if the plants are used as sentinels in the bioassay of mutagenic Cd
2+ and NaN
3. It was concluded that the
Vicia MCN bioassay should be used as an endpoint biomarker acceptable in biomonitoring environmental mutagens when the sentinel plants were collected from clean areas. Because no place is absolutely without pollutants, it is suggested that several seed stock centers should be established for providing sentinel
Vicia if
Vicia MCN bioassay is used as a biomarker to identify the global environmental status. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0098-8472 1873-7307 |
DOI: | 10.1016/S0098-8472(00)00058-7 |