Comparative mapping of genes from human chromosome 12 by genetic linkage mapping in cattle

• Published in: The Journal of heredity Vol. 90; no. 5; pp. 537 - 542
• Main Authors: Aleyasin, A, Barendse, W
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.09.1999; Oxford Publishing Limited (England)
• Subjects: Animals; Cattle; Cattle - genetics; chromosome 12; chromosome 17; chromosome 5; Chromosome Mapping; Chromosomes, Human, Pair 12; comparisons; Genes; Humans; LALBA gene; nucleotide sequences; PLA2G1B gene; Polymorphism, Genetic; sequence homology; SLC2A3 gene; SLC2A3L gene; Species Specificity; SYT1 gene; TPI1 gene
• ISSN: 0022-1503; 1465-7333
• DOI: 10.1093/jhered/90.5.537

Loci from human chromosome 12 were mapped in cattle to compare the gene order between species. Polymorphisms were detected in cattle in six loci that had been mapped with high precision in humans. Four of these loci, LALBA, SLC2A3, SYT1, and TPI1, mapped to bovine chromosome 5, and one, PLA2G1B, mapped to bovine chromosome 17. The sixth locus, SLC2A3L, due to a fragment produced by the SLC2A3 primers, maps to the telomeric region of BTA18. The differences in gene order between human chromosome 12 and cattle chromosome 5, when these loci are added to others already mapped in cattle, show evidence of significant rearrangement in gene order requiring several evolutionary events. There is also evidence in cattle chromosome 5 of the interspersal of material conserved on human chromosome 22 into the material conserved on human chromosome 12, consistent with ZOOFISH analyses. This analysis indicates that the larger block near the centromere is conserved on the long arm of human chromosome 12 and the smaller block near the telomere is conserved as part of the short arm of human chromosome 12. The level of variation detected in the amplified cattle DNA was approximately 1 variant per 464 nucleotides of haploid DNA using single-strand conformation polymorphism analysis. This corresponds to a per individual level of 1 variant per 1, 961 nucleotides of haploid DNA. This confirms lower genetic variability in cattle compared to humans but indicates the potential for millions of single nucleotide polymorphisms in cattle.