Application of solid waste from anaerobic digestion of poultry litter in Agrocybe aegerita cultivation: mushroom production, lignocellulolytic enzymes activity and substrate utilization

• Published in: Biodegradation (Dordrecht) Vol. 20; no. 3; pp. 351 - 361
• Main Authors: Isikhuemhen, Omoanghe S, Mikiashvili, Nona A, Kelkar, Vinaya
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.06.2009; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Agricultural wastes; Agrocybe - enzymology; Agrocybe - growth & development; Agrocybe aegerita; Anaerobic digestion; Anaerobiosis; Animals; Aquatic Pollution; Biodegradation; Biodegradation of pollutants; Biodegradation, Environmental; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Carbohydrates; Cellulase - metabolism; Cellulose; Cellulose 1,4-beta-Cellobiosidase - metabolism; Cultivation; Endo-1,4-beta Xylanases - metabolism; Environment and pollution; Enzymes; Fruiting Bodies, Fungal - chemistry; Fruiting Bodies, Fungal - growth & development; Fundamental and applied biological sciences. Psychology; Geochemistry; Glucan 1,4-beta-Glucosidase - metabolism; Industrial applications and implications. Economical aspects; Laccase - metabolism; Life Sciences; Lignin - metabolism; Litter; Manure; Manures; Microbiology; Millet; Mushrooms; Organic matter; Original Paper; Peroxidase - metabolism; Polysaccharides - metabolism; Poultry; Refuse Disposal - methods; Soil Science & Conservation; Solid wastes; Substrates; Terrestrial Pollution; Triticum aestivum; Waste Management/Waste Technology; Waste Water Technology; Water Management; Water Pollution Control; Wheat straw; Xylosidases - metabolism; Lignocellulose degradation; Anaerobic digester solids; Lignocellulolytic enzymes; Poultry litter; Sewage digester; Poultry; Lignocellulosics; Anaerobic digestion; Degradation; Fungi; Substrate; Enzymatic activity; Anaerobe; Production; Solid waste; Application; Agrocybe aegerita; Edible fungi; Culture
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-008-9226-y

The degradation and utilization of solid waste (SW) from anaerobic digestion of poultry litter by Agrocybe aegerita was evaluated through mushroom production, loss of organic matter (LOM), lignocellulolytic enzymes activity, lignocellulose degradation and mushroom nutrients content. Among the substrate combinations (SCs) tested, substrates composed of 10-20% SW, 70-80% wheat straw and 10% millet was found to produce the highest mushroom yield (770.5 and 642.9 g per 1.5 kg of substrate). LOM in all SCs tested varied between 8.8 and 48.2%. A. aegerita appears to degrade macromolecule components (0.6-21.8% lignin, 33.1-55.2% cellulose and 14-53.9% hemicellulose) during cultivation on the different SCs. Among the seven extracellular enzymes monitored, laccase, peroxidase and CMCase activities were higher before fruiting; while xylanase showed higher activities after fruiting. A source of carbohydrates (e.g., millet) in the substrate is needed in order to obtain yield and biological efficiency comparable to other commercially cultivated exotic mushrooms.