A sensitive method for the detection of mutagenic nitroarenes: construction of nitroreductase-overproducing derivatives of Salmonella typhimurium strains TA98 and TA100

• Published in: Mutation Research/Environmental Mutagenesis and Related Subjects Vol. 216; no. 4; pp. 211 - 220
• Main Authors: Watanabe, Masahiko, Ishidate, Motoi, Nohmi, Takehiko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.1989
• Subjects: Cloning; Cloning, Molecular; Cytosol; Genes, Bacterial; Mutagenecity; Mutagens - analysis; Nitro Compounds - analysis; Nitroarenes; Nitroreductase; Nitroreductases - biosynthesis; Nitroreductases - genetics; Oxidoreductases - biosynthesis; Plasmids; Restriction Mapping; Salmonella typhimurium; Salmonella typhimurium - enzymology; Species Specificity; Transformation, Bacterial; Nitroarenes; Salmonella typhimurium; Cloning; Mutagenecity; Nitroreductase

‘Classical nitroreductase’ is an enzyme involved in the intracellular metabolic activation of mutagenic nitroarenes. The nitroreductase gene of Salmonella typhimurium TA1538 was cloned into pBR322 and the plasmids harboring the gene were introduced into TA98 and TA100. The resulting strains (YG1021...