A sensitive method for the detection of mutagenic nitroarenes: construction of nitroreductase-overproducing derivatives of Salmonella typhimurium strains TA98 and TA100

‘Classical nitroreductase’ is an enzyme involved in the intracellular metabolic activation of mutagenic nitroarenes. The nitroreductase gene of Salmonella typhimurium TA1538 was cloned into pBR322 and the plasmids harboring the gene were introduced into TA98 and TA100. The resulting strains (YG1021...

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Published inMutation Research/Environmental Mutagenesis and Related Subjects Vol. 216; no. 4; pp. 211 - 220
Main Authors Watanabe, Masahiko, Ishidate, Motoi, Nohmi, Takehiko
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.08.1989
Subjects
Cloning
Cloning, Molecular
Cytosol
Genes, Bacterial
Mutagenecity
Mutagens - analysis
Nitro Compounds - analysis
Nitroarenes
Nitroreductase
Nitroreductases - biosynthesis
Nitroreductases - genetics
Oxidoreductases - biosynthesis
Plasmids
Restriction Mapping
Salmonella typhimurium
Salmonella typhimurium - enzymology
Species Specificity
Transformation, Bacterial
Nitroarenes
Salmonella typhimurium
Cloning
Mutagenecity
Nitroreductase
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Summary:‘Classical nitroreductase’ is an enzyme involved in the intracellular metabolic activation of mutagenic nitroarenes. The nitroreductase gene of Salmonella typhimurium TA1538 was cloned into pBR322 and the plasmids harboring the gene were introduced into TA98 and TA100. The resulting strains (YG1021 and YG1026) had more than 50 times higher nitrofurazone-reductase activity than TA1538 containing pBR322, and were extremely sensitive to the mutagenic action of 2-nitrofluorene, 1-nitropyrene and 2-nitronaphthalene. These results indicate that the new strains permit the efficient detection of mutagenic nitroarenes.
ISSN:0165-1161
0027-5107
DOI:10.1016/0165-1161(89)90007-1