Characterization of ADAM28 as a biomarker of bladder transitional cell carcinomas by urinary proteome analysis

• Published in: Biochemical and biophysical research communications Vol. 411; no. 4; pp. 714 - 720
• Main Authors: Yang, Ming-Hui, Chu, Pei-Yu, Chen, Sharon Chia-Ju, Chung, Tze-Wen, Chen, Wen-Cheng, Tan, Lia-Beng, Kan, Wei-Chih, Wang, Hsien-Yi, Su, Shih-Bin, Tyan, Yu-Chang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.08.2011
• Subjects: ADAM Proteins - metabolism; ADAM Proteins - urine; Aged; Aged, 80 and over; Amino Acid Sequence; Arsenic - urine; Biomarker; biomarkers; Biomarkers, Tumor - metabolism; bladder; Bladder cancer; Blotting, Western; carcinoma; Carcinoma, Transitional Cell - metabolism; Carcinoma, Transitional Cell - pathology; Carcinoma, Transitional Cell - urine; Female; Humans; Male; Middle Aged; Molecular Sequence Data; peptides; Protein identification; proteins; proteome; Proteome - metabolism; Proteomics; Taiwan; urinary bladder neoplasms; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology; Urinary Bladder Neoplasms - urine; Urinary protein; urinary tract diseases; urine; Western blotting; Taiwan; Protein identification; Biomarker; Bladder cancer; Urinary protein; Proteomics
• ISSN: 0006-291X; 1090-2104; 1090-2104
• DOI: 10.1016/j.bbrc.2011.07.010

► The aim of this study is to utilize proteomic approach to establish urinary protein patterns of bladder cancer. ► In this study, the arsenic concentrations in the urine of bladder cancer patients were still significantly higher than control group. ► Search for new biomarkers for the early detection, monitoring, and prognosis of bladder cancer is an active area of interest. ► The ADAM28 was confirmed by Western blotting and may be treated as possible biomarker of bladder cancer. Human urine contains a large number of proteins and peptides (the urinary proteome). Global analysis of the human urinary proteome is important for understanding urinary tract diseases. Bladder cancer is the most common urological cancer with higher incidence rates in endemic areas of Blackfoot disease (BFD) in southern Taiwan. The aim of this study was to use the proteomic approach to establish urinary protein biomarkers of bladder cancer. ADAM28, identified by proteomic approaches and confirmed by Western blotting, showed significant differences compared with normal individuals, so it may be a biomarker of bladder cancer.