Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (Eleusine indica L.)

• Published in: International journal of molecular sciences Vol. 24; no. 18; p. 13791
• Main Authors: Luo, Qiyu, Fu, Hao, Hu, Fang, Li, Shiguo, Chen, Qiqi, Peng, Shangming, Yang, Cunyi, Liu, Yaoguang, Chen, Yong
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.09.2023; MDPI
• Subjects: Abiotic stress; Ammonia; Crops; Enzymes; Gene expression; glufosinate resistance; Glutamine; goosegrass; herbicide stress; Herbicides; Ligases; Metabolism; Nitrogen; nitrogen metabolism; Plant genetics; Trends; Weeds; China; California; Malaysia
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241813791

Glufosinate is a broad-spectrum herbicide used to control most weeds in agriculture worldwide. Goosegrass (Eleusine indica L.) is one of the top ten malignant weeds across the world, showing high tolerance to glufosinate via different mechanisms that are not yet fully understood. This study revealed that nitrogen metabolism could be a target-resistant site, providing clues to finally clarify the mechanism of glufosinate resistance in resistant goosegrass populations. Compared to susceptible goosegrass (NX), the resistant goosegrass (AUS and CS) regarding the stress of glufosinate showed stronger resistance with lower ammonia contents, higher target enzyme GS (glutamine synthetase) activity, and lower GOGAT (glutamine 2-oxoglutarate aminotransferase) activity. The GDH (glutamate dehydrogenase) activity of another pathway increased, but its gene expression was downregulated in resistant goosegrass (AUS). Analyzing the transcriptome and proteome data of goosegrass under glufosinate stress at 36 h showed that the KEGG pathway of the nitrogen metabolism was enriched in glufosinate-susceptible goosegrass (NX), but not in glufosinate-resistant goosegrass (CS and AUS). Several putative target genes involved in glufosinate stress countermeasures were identified. This study provides specific insights into the nitrogen metabolism of resistant goosegrass, and gives a basis for future functional verification of glufosinate-tolerance genes in plants.