A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer

A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantifi...

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Bibliographic Details
Published inRSC advances Vol. 1; no. 14; pp. 887 - 892
Main Authors Lou, Ut Kei, Wong, Chi Hin, Chen, Yangchao
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 25.02.2020
The Royal Society of Chemistry
Subjects
Biomarkers
Cancer
Chemistry
Colorimetry
Diagnosis
Nanoparticles
Pancreatic cancer
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Summary:A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies. A colorimetric LAMP-(+)AuNP assay was proposed for detection of pancreatic cancer.
Bibliography:Electronic supplementary information (ESI) available: Details of Experimental section, tables and figures. See DOI
10.1039/c9ra07858d
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ISSN:2046-2069
2046-2069
DOI:10.1039/c9ra07858d