Biological Applications of Protein Splicing

Protein splicing is a naturally occurring process in which a protein editor, called an intein, performs a molecular disappearing act by cutting itself out of a host protein in a traceless manner. In the two decades since its discovery, protein splicing has been harnessed for the development of sever...

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Bibliographic Details
Published inCell Vol. 143; no. 2; pp. 191 - 200
Main Authors Vila-Perelló, Miquel, Muir, Tom W.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.10.2010
Subjects
Inteins
Protein Engineering - methods
Protein Processing, Post-Translational
Protein Splicing
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
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Summary:Protein splicing is a naturally occurring process in which a protein editor, called an intein, performs a molecular disappearing act by cutting itself out of a host protein in a traceless manner. In the two decades since its discovery, protein splicing has been harnessed for the development of several protein-engineering methods. Collectively, these technologies help bridge the fields of chemistry and biology, allowing hitherto impossible manipulations of protein covalent structure. These tools and their application are the subject of this Primer.
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ISSN:0092-8674
1097-4172
1097-4172
DOI:10.1016/j.cell.2010.09.031