Cloning and sequencing of the dnaK operon of Bacillus stearothermophilus

• Published in: Gene Vol. 170; no. 1; pp. 81 - 84
• Main Authors: Herbort, Markus, Schön, Ulrike, Angermann, Karin, Lang, Jens, Schumann, Wolfgang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 17.04.1996
• Subjects: Amino Acid Sequence; Bacillus stearothermophilus; Bacterial Proteins - genetics; Base Sequence; Chromosomes, Bacterial - genetics; Cloning, Molecular; DNA, Bacterial - genetics; dnaJ; Escherichia coli Proteins; Genetic Complementation Test; Geobacillus stearothermophilus - genetics; grpE; heat-shock protein; Heat-Shock Proteins - genetics; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins - genetics; molecular chaperone; Molecular Sequence Data; Open Reading Frames; Operon - genetics; Recombinant DNA; ORF; Bacillus subtilis; B. stearothermophilus; Escherichia coli; nt; kilobase(s) or 1000 bp; heat-shock protein; open reading frame; Recombinant DNA; denotes plasmid-carrier state; bp; nucleotide(s); kb; oligo; base pair(s); aa; HSP; amino acid(s); gene encoding GrpE; oligodeoxyribonucleotide; heat-shock protein(s); temperature sensitive; grpE; Bs; molecular chaperone; Bt; dnaK; dnaJ; gene encoding DnaJ; gene encoding DnaK; Ec; ts
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(95)00859-4

Here, we report the cloning of a 5.8-kb PstI fragment of chromosomal DNA from Bacillus stearothermophilus (Bt) carrying the three genes, grpE, dnaK and dnaJ. This fragment contains, in addition, the 3′-end of an open reading frame which has been shown to be part of the dnaK operon in three bacterial species. The dnaJ gene could complement an Escherichia coli dnaJts mutant for growth at high temperature. Sequencing and hybridization data strongly suggest that the Bt chromosome contains an analog of dnaJ.