Analyzing microglial-associated Aβ in Alzheimer's disease transgenic mice with a novel mid-domain Aβ-antibody

• Published in: Scientific reports Vol. 10; no. 1; p. 10590
• Main Authors: Henjum, Kristi, Årskog, Vibeke, Jendresen, Charlotte B, Fladby, Tormod, Torp, Reidun, Nilsson, Lars N G
• Format: Journal Article
• Language: English; Norwegian
• Published: England Nature Publishing Group 29.06.2020; Nature Publishing Group UK
• Subjects: Alzheimer Disease - immunology; Alzheimer Disease - pathology; Alzheimer's disease; Amino acids; Amyloid beta-Peptides - immunology; Amyloid beta-Peptides - metabolism; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - immunology; Amyloid beta-Protein Precursor - metabolism; Amyloid precursor protein; Amyloid Precursor Protein Secretases - metabolism; Animals; Antibodies; Antibodies - immunology; Disease Models, Animal; Epitopes; Humans; Immunoglobulin Domains - immunology; Macrophages; Mice; Mice, Transgenic; Microglia; Microglia - immunology; Microglia - physiology; Neurodegenerative diseases; Peptides; Plaque, Amyloid - metabolism; Senile plaques; Transgenic mice
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-67419-2

The mechanisms of amyloid-β (Aβ)-degradation and clearance in Alzheimer's disease (AD) pathogenesis have been relatively little studied. Short Aβ-fragments form by enzymatic cleavage and alternate amyloid-beta precursor protein (APP)-processing. Here we characterized a novel polyclonal Aβ-antibody raised against an Aβ mid-domain and used it to investigate microglial Aβ-uptake in situ by microscopy at the light- and ultrastructural levels. The rabbit Aβ-mid-domain antibody (ab338), raised against the mid-domain amino acids 21-34 (Aβ ), was characterized with biochemical and histological techniques. To identify the epitope in Aβ recognized by ab338, solid phase and solution binding data were compared with peptide folding scores as calculated with the Tango software. The ab338 antibody displayed high average affinity (K : 6.2 × 10  M) and showed preference for C-terminal truncated Aβ-peptides ending at amino acid 34 and Aβ-mid domain peptides with high scores of β-turn structure. In transgenic APP-mouse brain, ab338 labelled amyloid plaques and detected Aβ-fragments in microglia at the ultra- and light microscopic levels. This reinforces a role of microglia/macrophages in Aβ-clearance in vivo. The ab338 antibody might be a valuable tool to study Aβ-clearance by microglial uptake and Aβ-mid-domain peptides generated by enzymatic degradation and alternate production.