Pseudomonas aeruginosa: Mannose Sensitive Hemagglutinin Inhibits the Growth of Human Hepatocarcinoma Cells via Mannose-Mediated Apoptosis

• Published in: Digestive diseases and sciences Vol. 54; no. 10; pp. 2118 - 2127
• Main Authors: Cao, Zhenyuan, Shi, Lijun, Li, Ying, Wang, Jinghua, Wang, Dandan, Wang, Guangyou, Sun, Bo, Mu, Lili, Yang, Mingfei, Li, Hulun
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01.10.2009; Springer US; Springer; Springer Nature B.V
• Subjects: Apoptosis - drug effects; Bacterial Vaccines - immunology; Biochemistry; Biological and medical sciences; Carcinoma, Hepatocellular - immunology; Carcinoma, Hepatocellular - pathology; Cell Cycle; Feeding. Feeding behavior; Fimbria; Fimbriae Proteins - immunology; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Gastroenterology; Gastroenterology. Liver. Pancreas. Abdomen; Hemagglutinins - immunology; Hepatology; Humans; Immunologic Factors - immunology; In Situ Nick-End Labeling; Liver Neoplasms - immunology; Liver Neoplasms - pathology; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Mannose - pharmacology; Medical sciences; Medicine; Medicine & Public Health; Oncology; Original Article; Pseudomonas aeruginosa; Pseudomonas aeruginosa - immunology; Reverse Transcriptase Polymerase Chain Reaction; Transplant Surgery; Tumor Cells, Cultured; Tumors; Vertebrates: anatomy and physiology, studies on body, several organs or systems; PA-MSHA; Mannose; Hepatocarcinoma cells; Cell cycle; Apoptosis; Pseudomonadales; Human; Growth; Hepatic disease; Hepatocellular carcinoma; Metabolic diseases; Cell cycles; Malignant tumor; Gastroenterology; Bacteria; Digestive diseases; Pseudomonadaceae; Pseudomonas aeruginosa; Cancer
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-008-0603-5

A vaccine derived from the outer membrane proteins of the Gram-negative bacterium Pseudomonas aeruginosa has been shown to have immune modulatory properties. An inactivated mutant strain of P. aeruginosa with mannose sensitive hemagglutinin fimbria (PA-MSHA) has been used for adjuvant therapy for malignant cancer. In this study, the growth of human hepatocellular carcinoma Hep G2 and BEL-7402 cells is inhibited by PA-MSHA, but not by mannose-cleaved PA-MSHA. PA-MSHA-treated cells arrested in the S phase of the cell cycle and underwent apoptosis. We hypothesize that apoptosis induced by treatment of Hep G2 and BEL-7402 cells with PA-MSHA is mediated by the mannose residues of PA-MSHA and is propagated through the extrinsic apoptosis pathway directly through caspase-8. These data provide mechanistic details for the potential application of PA-MSHA-based treatment of hepatocellular carcinoma.