Analysis of epitope recognition of antibodies induced by DNA immunization against hemagglutinin protein of influenza A virus

In an effort to find efficient DNA vaccine candidates, cDNA of influenza A virus hemagglutinin (HA) gene and several derived mutants were injected into mice using a gene gun. Mice immunized with HA1 DNA, with or without a membrane domain, showed a humoral immune response and the survival rate agains...

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Published inVaccine Vol. 21; no. 23; pp. 3118 - 3125
Main Authors Tonegawa, Ken, Nobusawa, Eri, Nakajima, Katsuhisa, Kato, Takashi, Kutsuna, Takeo, Kuroda, Kazumichi, Shibata, Toshikatsu, Harada, Yuichi, Nakamura, Atsushi, Itoh, Makoto
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 04.07.2003
Elsevier
Elsevier Limited
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Summary:In an effort to find efficient DNA vaccine candidates, cDNA of influenza A virus hemagglutinin (HA) gene and several derived mutants were injected into mice using a gene gun. Mice immunized with HA1 DNA, with or without a membrane domain, showed a humoral immune response and the survival rate against homologous virus challenge was comparable to that of mice injected with HA DNA. In order to analyze epitopes recognized by antibodies induced by gene gun immunization, we used a binding assay employing the chimeric HA protein method. Serum antibodies of mice immunized with HA DNA recognized the HA1 domain but not the HA2 domain. In addition, antisera obtained from mice immunized with HA1 DNA reacted with each of the known antigenic sites on the HA1 domain, similar to the results obtained with HA DNA immunization.
Bibliography:ObjectType-Article-2
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content type line 23
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00257-3