The protective effect of dietary supplementation of Salmonella-specific bacteriophages in post-weaning piglets challenged with Salmonella typhimurium

• Published in: Journal of advanced veterinary and animal research Vol. 8; no. 3; pp. 440 - 447
• Main Authors: Won, Yong-Kwan, Kim, Sung-Jae, Han, Jeong-Hee
• Format: Journal Article
• Language: English
• Published: Bangladesh Network for the Veterinarians of Bangladesh Bangladesh Agricultural Universityת Faculty of Veterinary Science 01.09.2021; A periodical of the Network for the Veterinarians of Bangladesh (BDvetNET); Network for the Veterinarians of Bangladesh
• Subjects: Antibiotics; Antigens; Bacteria; bacteriophage; dietary supplement; s. typhimurium; piglets; diarrhea; growth performance; intestine; Cell density; Colonization; Crypts; Diarrhea; Diet; Dietary supplements; Drug resistance; Experiments; Feces; Feeds; Hogs; Infections; Inoculation; Intestine; Jejunum; Livestock; Necropsy; Original; Pathogens; Performance evaluation; Phages; Polymerase chain reaction; Salmonella; Small intestine; Swine; Villus; Weaning; S. typhimurium; growth performance; piglets; intestine; dietary supplement; Bacteriophage; diarrhea
• ISSN: 2311-7710; 2311-7710
• DOI: 10.5455/javar.2021.h532

The efficacy of specific bacteriophage STP-1 on infection in weaning piglets was evaluated in this study. Twenty-eight weaning piglets were randomly allocated to four groups (Group A: non-challenged/basal; Group B: non-challenged/+phage; Group C: challenged/basal; Group D: challenged/+phage) according to infection or bacteriophage administration. The total experimental period (14 days) was subdivided in to non-challenged periods (phase I; day 1-7) and challenged periods (phase II; day 7-14) based on the challenging date (day 7). Each group was fed with basal feed or feed supplemented with bacteriophage STP-1 [1.0 × 10 plaque-forming unit (PFU)/kg] during the whole period (day 1-14). Body weights (BW) were measured to evaluate growth performance. Clinical symptoms (rectal temperatures and fecal consistency) induced by were regularly checked. Bacteria colonization levels in feces and intestinal tissue samples were measured using real-time polymerase chain reaction (PCR). After necropsy, small intestine samples (jejunum) were collected. Villus height and crypt depth (CD) were measured through histological examination with H&E staining. The supplementation of bacteriophage significantly reduced bacterial colonization and intestine damage in the piglets infected with . In the antigen concentrations of the feces and jejunum, Group C showed 5.8 ± 0.6, 5.7 ± 0.6, and 1.2 ± 2.0 log colony-forming unit (CFU)/ml on 1, 3, and 7 days post-inoculation (DPI) and 2.8 ± 1.3 log CFU/ml, whereas Group D showed 3.5 ± 1.7, 2.2 ± 2.1, and 0.3 ± 0.9 log CFU/ml on 1, 3, and 7 DPI and 5.1 ± 0.9 log CFU/ml. In the villous height, Groups C and D showed 266.3 ± 24.1 and 324.6 ± 18.0 μm, respectively. In the goblet cell density of villi and crypts, Group C showed 10.0 ± 1.8 and 16.0 ± 3.7, while Group D showed 15.0 ± 4.8 and 21.1 ± 5.4. Also, the supplementation of bacteriophage significantly improved the growth performance in the infected piglets. The average daily gains of Groups C and D were 91 ± 24 and 143 ± 23, respectively, during the period after inoculation with . The dietary supplementation of the phage was effective for alleviating infection in post-weaning piglets.