rapid and easy to handle thermoluminescence based technique for evaluation of carbon tetrachloride-induced oxidative stress on rat hepatocytes

• Published in: Archives of toxicology Vol. 83; no. 7; pp. 709 - 720
• Main Authors: Schumann, Anika, Bauer, Alexander, Hermes, Matthias, Gilbert, Matthias, Hengstler, Jan G, Wilhelm, Christian
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.07.2009; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Carbon Tetrachloride - pharmacology; Carcinogens; Cell Culture Techniques; Cell Separation; Cell Survival - drug effects; Cells, Cultured; Coloring Agents - metabolism; Dose-Response Relationship, Drug; Environmental Health; Hepatocytes - drug effects; Hepatocytes - enzymology; Hepatocytes - metabolism; L-Lactate Dehydrogenase - analysis; Lipid Peroxidation - drug effects; Liver; Liver - cytology; Luminescence; Luminescent Measurements; Male; Malondialdehyde - metabolism; Medical sciences; Occupational Medicine/Industrial Medicine; Organ Toxicity and Mechanisms; Oxidative Stress - drug effects; Pharmacology/Toxicology; Rats; Rats, Sprague-Dawley; Rodents; Temperature; Thiobarbituric Acid Reactive Substances - analysis; Thiobarbituric Acid Reactive Substances - metabolism; Time Factors; Toxicology; Trypan Blue - metabolism; Secondary lipid peroxidation products; Rat hepatocytes; Carbon tetrachloride; Thermoluminescence; Evaluation; Oxidative stress; Secondary; Rat; Digestive system; Liver; Rodentia; Lipids; Vertebrata; Mammalia; Hepatocyte; Animal; Technique; Peroxidation
• ISSN: 0340-5761; 1432-0738
• DOI: 10.1007/s00204-009-0404-4

Oxidative stress has become one of the most intensively studied topics in biomedical research and is an often observed mechanism of non-genotoxic carcinogens like carbon tetrachloride. To monitor the oxidative stress status in in vitro hepatocytes, we compared thermoluminescence (TL) measurements with biochemical standard methods for oxidative stress markers. In contrast to biochemical analysis, TL measurements can be performed without any time-consuming extraction procedures by using directly collected cell material. After incubation with CCl₄ (24 h), thermo-induced light emission increased with rising concentration of CCl₄ up to eightfold at 10 mM CCl₄. Simultaneously, we determined the content of different secondary oxidative stress products, like thiobarbituric acid reactive substances and malondialdehyde. The rise of all biochemical markers complied with the increasing concentration of CCl₄. Finally, we could show that the CCl₄-induced increase of oxidative stress markers determined by time-consuming biochemical methods perfectly correlates with the increase of high temperature bands in rapid TL measurements.