Characterisation of micromonosporae from aquatic environments using molecular taxonomic methods

• Published in: Antonie van Leeuwenhoek Vol. 94; no. 2; pp. 289 - 298
• Main Authors: Maldonado, Luis A, Stach, James E. M, Ward, Alan C, Bull, Alan T, Goodfellow, Michael
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.08.2008; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Aquatic ecosystems; Aquatic environment; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Biomedical and Life Sciences; classification; DNA Fingerprinting; DNA Fingerprinting - methods; DNA, Bacterial; DNA, Bacterial - genetics; DNA, Ribosomal; DNA, Ribosomal - genetics; ecosystems; Fundamental and applied biological sciences. Psychology; genetics; Geologic Sediments; Geologic Sediments - microbiology; isolation & purification; Life Sciences; Medical Microbiology; methods; Microbiology; Micromonospora; Micromonospora - classification; Micromonospora - genetics; Micromonospora - isolation & purification; Molecular biology; Molecular Sequence Data; nucleotide sequences; Numerical analysis; Original Paper; Phylogeny; Pigmentation; Plant Sciences; Ribonucleic acid; ribosomal RNA; RNA; RNA, Ribosomal, 16S; RNA, Ribosomal, 16S - genetics; sediments; sequence analysis; Sequence Analysis, DNA; Soil Science & Conservation; Systematics; Taxonomy; Water Microbiology; Marine environment; Molecular fingerprinting; 16S rDNA sequencing; PCR; Micromonosporaceae; Micromonospora; Ribosomal DNA; Actinomycetales; Polymerase chain reaction; rep-PCR; Aquatic environment; Fingerprint method; Bacteria; Environment; Actinomycetes
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-008-9244-0

Large numbers of strains assigned to the genus Micromonospora on the basis of typical colonial and pigmentation features were isolated from diverse aquatic sediments using a standard selective isolation procedure. Two hundred and six isolates and eight representatives of the genus Micromonospora were assigned to 24 multimembered groups based on a numerical analysis of banding patterns generated using BOX and ERIC primers. Representatives of multimembered groups encompassing isolated micromonosporae were the subject of 16S rRNA gene sequencing analyses. Good congruence was found between the molecular fingerprinting and 16S rRNA sequence data indicating that the groups based upon the former are taxonomically meaningful. Nearly all of the isolates that were chosen for the 16S rRNA gene sequencing analyses showed that the ecosystems studied are a rich source of novel micromonosporae. These findings have implications for high throughput screening for novel micromonosporae as BOX and ERIC fingerprinting, which is rapid and reproducible, can be applied as a robust dereplication procedure to indicate which environmental isolates have been cultured previously.