Simultaneous determination of six main nucleosides and bases in natural and cultured Cordyceps by capillary electrophoresis

• Published in: Journal of Chromatography A Vol. 1055; no. 1; pp. 215 - 221
• Main Authors: Gong, Y.X., Li, S.P., Li, P., Liu, J.J., Wang, Y.T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05.11.2004; Elsevier
• Subjects: Analysis; Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Buffers; Central composite design; Chemistry; Chromatographic methods and physical methods associated with chromatography; Cordyceps; Cordyceps - chemistry; Determination; Electrophoresis, Capillary - methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General pharmacology; Hydrogen-Ion Concentration; Medical sciences; Nucleosides - analysis; Other chromatographic methods; Pharmacology. Drug treatments; Quality control; Reference Standards; Central composite design; Determination; Cordyceps; Quality control; Capillary electrophoresis; Quality control: Determination; Composite material; Fungi; Design; Microbial origin; Simultaneous measurement; Nucleoside; Fungi Imperfecti; Thallophyta
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.09.015

A simple method is described for simultaneous determination of six main nucleosides and bases including adenine, uracil, adenosine, guanosine, uridine and inosine in Cordyceps by capillary electrophoresis (CE). Chemometric optimization based on central composite design was employed to find the optimum resolution. The optimum factor space was defined by three parameters: buffer concentration, pH and concentration of acetonitrile as organic modifier. Resolution ( R S) was employed to evaluate the response function. A running buffer composed of 500 mM boric acid, adjusted pH to 8.6 with sodium hydroxide and 12.2% acetonitrile as modifier was found to be the most appropriate for the separation. The contents of the six components were determined by using adenosine monophosphate as an internal standard. Furthermore, hierarchical clustering analysis based on characteristics of 32 peaks in CE profiles from the tested 12 samples showed that natural and cultured Cordyceps were in different clusters. Adenosine and inosine were extracted as markers for discrimination of natural Cordyceps. The result of clustering based on the two peaks characteristics was in excellent agreement with that based on 32 peaks’. Thus, adenosine and inosine could be used as markers for quality control of natural and cultured Cordyceps.