Reversed-Phase HPLC Determination of Eight Anticoagulant Rodenticides in Animal Liver

A reversed-phase high-performance liquid chromatographic method was developed for the analysis of eight anticoagulant rodenticides in animal liver. Coumarinic anticoagulant rodenticides (brodifacoum, bromadiolone, coumachlor, coumatetralyl, difenacoum, and warfarin) were detected by using a gradient...

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Published inJournal of analytical toxicology Vol. 21; no. 7; pp. 548 - 553
Main Authors Fauconnet, V., Pouliquen, H., Pinault, L.
Format Journal Article
LanguageEnglish
Published Niles, IL Oxford University Press 01.11.1997
Preston
Subjects
Animals
Anticoagulants - analysis
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid
Coumarins - analysis
Liver - chemistry
Medical sciences
Pesticides, fertilizers and other agrochemicals toxicology
Rats
Rodenticides - analysis
Solvents
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Toxicology
Solid phase extraction
Fluorescence detector
Elution
Gradient
Chemical analysis
Coumarine derivatives
Liver
Indanedione derivatives
Pesticides
HPLC chromatography
Anticoagulant
Isocratic condition
Rodenticide
Biological material
Reversed phase chromatography
Multicomponent analysis
Ultraviolet detector
Sample preparation
Animal
Simultaneous measurement
Quantitative analysis
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Summary:A reversed-phase high-performance liquid chromatographic method was developed for the analysis of eight anticoagulant rodenticides in animal liver. Coumarinic anticoagulant rodenticides (brodifacoum, bromadiolone, coumachlor, coumatetralyl, difenacoum, and warfarin) were detected by using a gradient elution and a fluorimetric detection. Indanedione anticoagulant rodenticides (chlorophacinone and diphacinone) were detected by using an isocratic elution and an UV detection. Anticoagulants were extracted from liver with mixtures of acetone/diethylether and acetone/chloroform. Extracts were applied to solid-phase extraction cartridges. Linearity was checked over the concentration range 0.1–0.6 µg/g. Relative standard deviations of within-run and between-run variability were all between 5.7 and 10.3%. Recoveries from spiked liver samples were between 51.7 (difenacoum) and 78.2% (warfarin). Limits of detection were between 0.01 (difenacoum and warfarin) and 0.11 µg/g (chlorophacinone).
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Author to whom correspondence should be addressed.
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ISSN:0146-4760
1945-2403
DOI:10.1093/jat/21.7.548