Sequence Analysis of Major Histocompatibility Complex Class-II DQB1 ( Patr-DQB1) Alleles in Chimpanzees by Polymerase Chain Reaction–Based Methods

• Published in: Human Immunology Vol. 67; no. 8; pp. 655 - 663
• Main Authors: Bak, EunJung, Ishii, Yoshiyuki, Omatsu, Tsutomu, Kyuwa, Shigeru, Tanoue, Tetsuya, Hayasaka, Ikuo, Yoshikawa, Yasuhiro
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2006; Elsevier BV
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Female; Histocompatibility Antigens Class II; Histocompatibility Antigens Class II - genetics; Male; Molecular Sequence Data; Pan troglodytes; Pan troglodytes - genetics; Pan troglodytes - immunology; Patr-DQB1 allele; PCR-RFLP; Polymerase Chain Reaction; Primates; sequence analysis; Sequence Analysis, DNA; Japan; Patr-DQB1 allele; sequence analysis; PCR-RFLP
• ISSN: 0198-8859; 1879-1166; 1365-2567
• DOI: 10.1016/j.humimm.2006.04.016

The major histocompatibility complex (MHC) class-II genes are described as the genes that encode the antigen-presenting molecule. In particular, functional alleles of MHC class-II genes in nonhuman primates have been analyzed as part of the study of infectious and immune-mediated diseases in animal models and as part of efforts to understand the molecular evolution of human leukocyte antigen. The polymorphisms and sequence analysis of MHC class-II genes in a large number of subjects is necessary for the group management of nonhuman primates. In the present study, we attempted to analyze the DQB1 polymorphism in the common chimpanzee ( Pan troglodytes) by exon 2 sequencing. For exact typing of Patr-DQB1 alleles, we carried out polymerase chain reaction–restriction fragment length polymorphism and sequence analysis. In the genomic DNA of 25 chimpanzees, 6 Patr-DQB1 alleles, including 2 new alleles, were detected. Identification and analysis of Patr-DQB1 alleles using this method may contribute to our understanding of Patr-DQB1 allelic diversity in individual chimpanzees and should be useful in facilitating colony management of chimpanzee groups in Japan.