Signal molecules in the peanut-bradyrhizobia interaction

• Published in: Archives of microbiology Vol. 189; no. 4; pp. 345 - 356
• Main Authors: Taurian, Tania, Morón, Belén, Soria-Díaz, María E, Angelini, Jorge G, Tejero-Mateo, Pilar, Gil-Serrano, Antonio, Megías, Manuel, Fabra, Adriana
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2008; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Arachis - chemistry; Arachis - microbiology; Arachis - physiology; Arachis hypogaea; Bacteria; Bacteriology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Bradyrhizobium; Bradyrhizobium - chemistry; Bradyrhizobium - classification; Bradyrhizobium - genetics; Bradyrhizobium - physiology; Cell Biology; Chromatography; Cultivars; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Ecology; Flavonoids; Flavonoids - chemistry; Flavonoids - metabolism; Fundamental and applied biological sciences. Psychology; Genes; Infections; Legumes; Life Sciences; Lipopolysaccharides - chemistry; Lipopolysaccharides - metabolism; Mass spectrometry; Microbial Ecology; Microbiology; Miscellaneous; Molecular Sequence Data; Original Paper; Peanuts; Phylogeny; Rhizobium; Ribosomal DNA; RNA, Ribosomal, 16S - genetics; Root Nodules, Plant - chemistry; Root Nodules, Plant - metabolism; Root Nodules, Plant - microbiology; Scientific imaging; Sequence Analysis, DNA; Soil Microbiology; Species Specificity; Symbiosis; Thin layer chromatography; Spain; Flavonoids; Nodulation and fixation genes; Rhizobial isolates; L; Nod factors; Symbiosis; Arachis hypogaea; Nodulation; Rhizobium; Arachis hypogaea L; Symbiont; Flavonoid; Polyphenol; Fixation; Leguminosae; Gene; Dicotyledones; Angiospermae; Phenols; Bacteria; Isolate; Spermatophyta; Rhizobiaceae; Oil plant (vegetal); Bradyrhizobium
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-007-0325-7

Main nodulation signal molecules in the peanut-bradyrhizobia interaction were examined. Flavonoids exuded by Arachis hypogaea L. cultivar Tegua were genistein, daidzein and chrysin, the latest being released in lower quantities. Thin layer chromatography analysis from genistein-induced bacterial cultures of three peanut bradyrhizobia resulted in an identical Nod factor pattern, suggesting low variability in genes involved in the synthesis of these molecules. Structural study of Nod factor by mass spectrometry and NMR analysis revealed that it shares a variety of substituents with the broad-host-range Rhizobium sp. NGR234 and Bradyrhizobium spp. Nodulation assays in legumes nodulated by these rhizobia demonstrated differences between them and the three peanut bradyrhizobia. The three isolates were classified as Bradyrhizobium sp. Their fixation gene nifD and the common nodulation genes nodD and nodA were also analyzed.