Analysis of the ontogeny of galanin in the rat central nervous system by immunohistochemistry and radioimmunoassay

• Published in: International journal of developmental neuroscience Vol. 8; no. 1; p. 81
• Main Authors: Sizer, A R, Rökaeus, A, Foster, G A
• Format: Journal Article
• Language: English
• Published: United States 1990
• Subjects: Aging - metabolism; Animals; Central Nervous System - growth & development; Central Nervous System - metabolism; Galanin; Immunohistochemistry; Peptides - metabolism; Peptides - physiology; Radioimmunoassay; Rats
• ISSN: 0736-5748
• DOI: 10.1016/0736-5748(90)90025-W

The development of galanin-like immunoreactivity in the rat central nervous system has been investigated immunologically. Galanin-positive processes in the central nervous system were first recognized at day 1 post-natal, in the dorsal horn of the spinal cord. At day 2, the first galanin-immunoreactive neuronal somata were visualized in several regions of the diencephalon; at later stages of maturation positive cells were also detected in many brain stem areas. The number, density and staining intensity of galanin-positive structures in these and other regions increased steadily until day 28, by which age the adult disposition was attained. Increases in the concentrations of galanin-like immunoreactivity during maturation of the animals closely paralleled the immunohistochemical findings. No reduction in galanin-like immunoreactivity was noted in any area during later post-natal ages. The present study indicates that the ontogeny of galanin-like immunoreactivity in the rat central nervous system occurs entirely post-natally. The developmental profile is consistent with the role of galanin as a putative neurotransmitter/neuromodulator in the rat brain and spinal cord.