Sample preparation and culture condition effects on MALDI‐TOF MS identification of bacteria: A review

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environmental research. Currently, there is no optimal standardize...

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Published inMass spectrometry reviews Vol. 42; no. 5; pp. 1589 - 1603
Main Authors Topić Popović, Natalija, Kazazić, Snježana P., Bojanić, Krunoslav, Strunjak‐Perović, Ivančica, Čož‐Rakovac, Rozelindra
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.09.2023
Subjects
bacteria
culture conditions
Culture media
diagnostics
Growth stage
MALDI‐TOF MS
Mass spectroscopy
sample preparation
MALDI-TOF MS
diagnostics
culture conditions
bacteria
sample preparation
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Summary:Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environmental research. Currently, there is no optimal standardized protocol for sample preparation and culture conditions to profile bacteria. The performance of MALDI‐TOF MS is affected by several variables, such as sample preparation, culture media and culture conditions, incubation time/growth stage, incubation temperature, high salt content, blood in the culture media, and others. This review thus aims to clarify why a uniformed protocol is not plausible, to assess the effects these factors have on MALDI‐TOF MS identification score, and discuss possible optimizations for its methodology, in relation to specific bacterial representatives and strain requirements.
Bibliography:Natalija Topić Popović and Snježana P. Kazazić have contributed equally to this study.
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ISSN:0277-7037
1098-2787
DOI:10.1002/mas.21739