Establishment of practical recellularized liver graft for blood perfusion using primary rat hepatocytes and liver sinusoidal endothelial cells

• Published in: American journal of transplantation Vol. 18; no. 6; pp. 1351 - 1359
• Main Authors: Kojima, H., Yasuchika, K., Fukumitsu, K., Ishii, T., Ogiso, S., Miyauchi, Y., Yamaoka, R., Kawai, T., Katayama, H., Yoshitoshi‐Uebayashi, E. Y., Kita, S., Yasuda, K., Sasaki, N., Komori, J., Uemoto, S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Limited 01.06.2018
• Subjects: animal models: murine; Animals; artificial organs/support devices; basic (laboratory) research/science; Blood; Cells, Cultured; cellular biology; Clotting; Endothelial cells; Endothelial Cells - cytology; Hepatocytes; Hepatocytes - cytology; Liver; Liver - cytology; Liver - physiology; Liver Transplantation; liver transplantation/hepatology; Liver transplants; Male; Parenchyma; Perfusion; Portal vein; Rats; Rats, Inbred Lew; Rats, Transgenic; tissue/organ engineering; animal models: murine; cellular biology; artificial organs/support devices; liver transplantation/hepatology; basic (laboratory) research/science; tissue/organ engineering
• ISSN: 1600-6135; 1600-6143
• DOI: 10.1111/ajt.14666

Tissue decellularization produces a three‐dimensional scaffold that can be used to fabricate functional liver grafts following recellularization. Inappropriate cell distribution and clotting during blood perfusion hinder the practical use of recellularized livers. Here we aimed to establish a seeding method for the optimal distribution of parenchymal and endothelial cells, and to evaluate the effect of liver sinusoidal endothelial cells (LSECs) in the decellularized liver. Primary rat hepatocytes and LSECs were seeded into decellularized whole‐liver scaffolds via the biliary duct and portal vein, respectively. Biliary duct seeding provided appropriate hepatocyte distribution into the parenchymal space, and portal vein–seeded LSECs simultaneously lined the portal lumen, thereby maintaining function and morphology. Hepatocytes co‐seeded with LSECs retained their function compared with those seeded alone. Platelet deposition was significantly decreased and hepatocyte viability was maintained in the co‐seeded group after extracorporeal blood perfusion. In conclusion, our seeding method provided optimal cell distribution into the parenchyma and vasculature according to the three‐dimensional structure of the decellularized liver. LSECs maintained hepatic function, and supported hepatocyte viability under blood perfusion in the engineered liver graft owing to their antithrombogenicity. This recellularization procedure could help produce practical liver grafts with blood perfusion. The authors provide a seeding method for achieving optimal cell distribution in the parenchyma and vasculature of the decellularized liver, and evaluate the effect of endothelial cells in the engineered liver.