A combination of resveratrol and quercetin induces browning in white adipose tissue of rats fed an obesogenic diet

• Published in: Obesity (Silver Spring, Md.) Vol. 25; no. 1; pp. 111 - 121
• Main Authors: Arias, Noemí, Picó, Catalina, Teresa Macarulla, M., Oliver, Paula, Miranda, Jonatan, Palou, Andreu, Portillo, María P.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.01.2017
• Subjects: Adipocytes; Adipocytes - drug effects; Adipocytes - metabolism; Adipose Tissue, Brown - drug effects; Adipose Tissue, Brown - physiology; Adipose Tissue, White - drug effects; Adipose Tissue, White - physiology; Amino Acid Transport Systems, Neutral - genetics; Amino Acid Transport Systems, Neutral - metabolism; Animals; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; Body fat; Body Weight; Bone Morphogenetic Protein 4 - genetics; Bone Morphogenetic Protein 4 - metabolism; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Deoxyribonucleic acid; Diet; DNA; Fatty Acid-Binding Proteins - genetics; Fatty Acid-Binding Proteins - metabolism; Fatty acids; Gene expression; Homeostasis; Lipase - genetics; Lipase - metabolism; Mitochondrial DNA; Morphology; Obesity; Oxidation; Polyphenols; Protein expression; Proteins; Quercetin - pharmacology; Rats; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Stilbenes - pharmacology; Studies; Symporters - genetics; Symporters - metabolism; Thermogenesis; Uncoupling Protein 1 - genetics; Uncoupling Protein 1 - metabolism
• ISSN: 1930-7381; 1930-739X
• DOI: 10.1002/oby.21706

Objective To analyze whether a combination of quercetin (Q) and resveratrol (RSV) would induce a white adipose tissue (WAT) browning effect. Methods Thirty‐six rats were fed an obesogenic diet and divided into four groups: control, treated with RSV (15 mg/kg body weight/day; RSV group), treated with Q (30 mg/kg body weight/day; Q group), or treated with both polyphenols (RSV + Q group). Results After 6 weeks, body and WAT weights were significantly reduced in the RSV + Q group. In perirenal WAT of the control, RSV, and Q groups, white unilocular adipocytes appeared in the majority of cells, while in the RSV + Q group numerous multilocular adipocytes with positive immunostaining for UCP1 were observed. The presence of UCP1 was confirmed by Western blot. This group also revealed increased mRNA levels of Cidea, Hocx9, Bmp4, Slc27a1, Pat2, Atgl, and Atp5d. Interscapular brown adipose tissue weight showed no differences between groups, but the Cidea mRNA level was increased in the RSV group, the Cox‐2 mRNA level in the RSV + Q group, and UCP1 protein expression in the RSV and the RSV + Q groups. Conclusions This study demonstrated that the RSV + Q combination produces a brown‐like remodeling effect in perirenal WAT, as well as increased UCP1 protein expression in interscapular brown adipose tissue.