Cross‐talk between Tor1 and Sch9 regulates hyphae‐specific genes or ribosomal protein genes in a mutually exclusive manner in Candida albicans

• Published in: Molecular microbiology Vol. 112; no. 3; pp. 1041 - 1057
• Main Authors: Kim, Se Woong, Joo, Yoo Jin, Chun, Yu Jin, Park, Young Kwang, Kim, Joon
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.09.2019
• Subjects: Candida albicans; Candida albicans - enzymology; Candida albicans - genetics; Candida albicans - growth & development; Cascades; Cyclic AMP; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Expression Regulation, Fungal; Genes; Hyphae; Hyphae - enzymology; Hyphae - genetics; Hyphae - growth & development; Kinases; Morphogenesis; Morphology; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - metabolism; Protein kinase A; Protein Kinases - genetics; Protein Kinases - metabolism; Proteins; Ribosomal proteins; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; Switches; Transcription; Yeast; Yeasts
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1111/mmi.14346

Summary The human fungal pathogen Candida albicans switches its morphology from yeast to hyphal forms. The morphological transition may render C. albicans virulent. Several signaling cascades, including those of the cyclic AMP‐protein kinase A and mitogen‐activated protein kinase pathways, are responsible for morphogenesis. In this study, we observed a reduction in gene transcription of ribosomal proteins during true hyphae formation. Moreover, morphogenesis‐dependent decrease in ribosomal protein gene transcription was confirmed in constitutive yeast or filamentous growing strains. We consistently observed that polysome and monosome levels were decreased by hyphal stimuli through TORC1 and Sch9 kinases. Taken together, these results provide several lines of evidence to show that the Tor1–Sch9 kinase cascade, which stimulates transcription of ribosomal protein genes, exists in C. albicans. Thus, the present study revealed a novel link between ribosome biogenesis and morphogenesis in C. albicans that is mediated by Tor1 and Sch9. Tor1 – Sch9 kinase signaling cascade mediates stimulation of ribosomal protein genes transcription and inhibition of adhesion genes transcription in a mutually exclusive manner in Candida albicans.