Serological evidence of human infection by bat orthoreovirus in Singapore

• Published in: Journal of medical virology Vol. 91; no. 4; pp. 707 - 710
• Main Authors: Uehara, Anna, Tan, Chee Wah, Mani, Shailendra, Chua, Kaw Bing, Leo, Yee Sin, Anderson, Danielle E, Wang, Lin‐Fa
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2019
• Subjects: Adult; Aged; Aged, 80 and over; Animals; Antibodies; Antibodies, Viral - blood; bat; Female; Human populations; Humans; Immunoprecipitation; Longitudinal Studies; luciferase immunoprecipitation system (LIPS); Male; Middle Aged; Neutralization; orthoreovirus; Orthoreovirus - immunology; Reoviridae Infections - epidemiology; Reoviridae Infections - virology; Seroepidemiologic Studies; Serologic Tests - methods; Singapore - epidemiology; spillover; Virology; virus neutralization test (VNT); Viruses; Young Adult; Zoonoses - epidemiology; Zoonoses - virology; Singapore; spillover; bat; luciferase immunoprecipitation system (LIPS); orthoreovirus; virus neutralization test (VNT)
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.25355

To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected from 2005‐2013. After an initial screen with luciferase immunoprecipitation system and secondary confirmation with virus neutralization test, we identified at least seven individuals with specific antibodies against PRV in both assays. Our findings confirm that PRV spillover into human populations is relatively common in this region of the world.