OsRAN2, essential for mitosis, enhances cold tolerance in rice by promoting export of intranuclear tubulin and maintaining cell division under cold stress

• Published in: Plant, cell and environment Vol. 34; no. 1; pp. 52 - 64
• Main Authors: CHEN, NA, XU, YUNYUAN, WANG, XIN, DU, CHENG, DU, JIZHOU, YUAN, MING, XU, ZHIHONG, CHONG, KANG
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 2011; Blackwell
• Subjects: Adaptation, Physiological; Amino Acid Sequence; Arabidopsis - genetics; Biological and medical sciences; Cell Division - genetics; Cold Temperature - adverse effects; cold tolerance; DNA, Plant - genetics; exports; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene Expression Regulation, Plant; Immunohistochemistry; intact nuclear envelope; Meristem - cytology; Meristem - ultrastructure; Microscopy, Electron, Transmission; Microtubules - metabolism; Mitosis - genetics; Nuclear Envelope - metabolism; Oryza - genetics; Oryza - metabolism; Oryza - physiology; Oryza - ultrastructure; Oryza sativa; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Proteins - physiology; Plants, Genetically Modified; ran GTP-Binding Protein - genetics; ran GTP-Binding Protein - metabolism; ran GTP-Binding Protein - physiology; RNA, Plant - genetics; Salts - adverse effects; small GTPase; Stress; Stress, Physiological; Tubulin - metabolism; Up-Regulation; Monocotyledones; Mitosis; Cold resistance; Plant ecology; Cell division; Tolerance; intact nuclear envelope; Nuclear envelope; Stress; Oryza; Tubulin; Gramineae; Angiospermae; Export; Herbaceous plant; Spermatophyta; Cold induced disorder; small GTPase
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/j.1365-3040.2010.02225.x

With global climate change, abnormally low temperatures have affected the world's rice production. Many genes have been shown to be essential for molecular improvement of rice cold-tolerance traits. However, less is known about the molecular cellular mechanism of their response to cold stress. Here, we investigated OsRAN2 involved in regulation of cell division during cold stress in rice. Expression of OsRAN2 was increased under cold treatment, but not during salt and drought stress. The mean root mitotic index was closely related to the expression level of OsRAN2. Knockdown transgenic rice lines showed an aberrant organization of spindles during mitosis and stunted growth during development. Overexpression of OsRAN2 enhanced cold tolerance in rice. The transgenic rice overexpressing OsRAN2 showed maintained cell division, decreased proportion of cells with intranuclear tubulin and formation of a normal nuclear envelope under the cold condition. Our study suggests a mechanism for OsRAN2 in regulating cold resistance in rice by maintaining cell division through promoting the normal export of intranuclear tubulin at the end of mitosis. This insight could help improve the cold-tolerance trait in rice.