Toxicological comparison of diverse Cylindrospermopsis raciborskii strains: Evidence of liver damage caused by a French C. raciborskii strain

• Published in: Environmental toxicology Vol. 18; no. 3; pp. 176 - 186
• Main Authors: Bernard, C., Harvey, M., Briand, J. F., Biré, R., Krys, S., Fontaine, J. J.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 2003; Wiley
• Subjects: Animals; Bacteriology; Biological and medical sciences; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - pathology; Chromatography, High Pressure Liquid; Cyanobacteria - classification; Cyanobacteria - cytology; Cyanobacteria - physiology; cyanobacterium; Cyanophyta; cylindrospermopsin; Cylindrospermopsis raciborskii; decarbamoylsaxitoxin; Environmental pollutants toxicology; Freshwater; Fundamental and applied biological sciences. Psychology; health effect; hepatic toxicity; Life Sciences; Liver - drug effects; Liver - pathology; Liver Diseases; Male; Marine Toxins - chemistry; Marine Toxins - toxicity; Mass Spectrometry; Medical sciences; Mice; Microbiology; neosaxitoxin; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Raphidiopsis sp; renal toxicity; saxitoxin; Saxitoxin - toxicity; Species Specificity; Toxicology; Water; Water Microbiology; France; Europe; Hungary; USA, Florida; Brazil; Mexico; Thailand; Australia; renal toxicity; cylindrospermopsin; decarbamoylsaxitoxin; Digestive system; Toxicity; Cylindrospermopsis raciborskii; Liver; health effect; neosaxitoxin; Kidney; hepatic toxicity; Toxin; Health status; Urinary system; Cyanobacteria; Bacteria; cyanobacterium; Damage; Raphidiopsis sp; saxitoxin; Public health
• ISSN: 1520-4081; 1522-7278
• DOI: 10.1002/tox.10112

The freshwater cyanobacterium Cylindrospermopsis raciborskii is known to produce toxic effects in several countries. Acute and chronic exposures to C. raciborskii in Australia have been linked to liver damage (hepatotoxicity) with concomitant effects on the kidneys, adrenal glands, small intestine, lungs, thymus, and heart. The alkaloid cylindrospermopsin, which produces these toxic effects, is thought to be a potent inhibitor of protein synthesis. C. raciborskii strains producing cylindrospermopsin or analogue alkaloids have also been reported in Florida, USA, and Thailand. Brazilian isolates of C. raciborskii are also toxic but act by a different mechanism, causing acute death in mice with neurotoxic symptoms similar to those induced by the saxitoxins. In this article we compare the toxicity in the mouse of a C. raciborskii French strain with C. raciborskii strains from various other sources (Australia, Brazil, Mexico, and Hungary). We tested the toxicity of cell extracts by a mouse bioassay. Acute, fatal neurotoxicity was produced by the Brazilian strain, which was confirmed by liquid chromatography with fluorescence detection of the cell extracts, which revealed the presence of saxitoxin, neosaxitoxin, and decarbamoylsaxitoxin, along with two unidentified compounds. Acute hepatotoxicity with severe liver, kidney, and thymus damage was observed with the Australian cylindrospermopsin‐producing strain. The Mexican and Hungarian strains were not found to be toxic to mice in our experimental conditions. No animals died after exposure to the extracts of the French C. raciborskii strain. Histological examination of the liver revealed moderate, multifocal necrosis characterized by small areas of hepatocellular necrosis, combined with disorganization of the parenchyma and congestion of the inner sinusoid. These symptoms and lesions resembled those induced by cylindrospermopsin, but the chemical analysis performed by liquid chromatography coupled with either a diode array detector or a mass spectrometer demonstrated that this toxin was not present in our culture extract. © 2003 Wiley Periodicals, Inc. Environ Toxicol 18: 176–186, 2003.