Rapid and highly sensitive liquid chromatography/electrospray ionization tandem mass spectrometry method for the quantitation of buspirone in human plasma: application to a pharmacokinetic study

• Published in: Rapid communications in mass spectrometry Vol. 20; no. 8; pp. 1293 - 1298
• Main Authors: Cho, Sung-Hee, Lee, Heon-Woo, Im, Ho-Taek, Park, Wan-Su, Choi, Young-Wook, Rew, Jae-Hwan, Lee, Kyung-Tae
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.01.2006
• Subjects: Adult; Amlodipine - blood; Anti-Anxiety Agents - blood; Anti-Anxiety Agents - pharmacokinetics; Area Under Curve; Buspirone - blood; Buspirone - pharmacokinetics; Calcium Channel Blockers - blood; Calibration; Chromatography, Liquid; Half-Life; Humans; Indicators and Reagents; Male; Quality Control; Reference Standards; Reproducibility of Results; Solvents; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry
• ISSN: 0951-4198; 1097-0231
• DOI: 10.1002/rcm.2441

A rapid and sensitive method for the quantitation of buspirone in human plasma by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) was developed. Plasma samples were treated by liquid‐liquid extraction with methyl tert‐butyl ether (MTBE). The chromatographic separation was performed isocratically on a reversed‐phase Shiseido C18 column (50 mm × 2.0 mm, 3 µm) with a mobile phase of acetonitrile/0.1% acetic acid (1:1, v/v). The acquisition was performed in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 386 → 122 for buspirone and m/z 409 → 238 for amlodipine (the internal standard). The method was validated to determine its specificity, recovery, limit of quantitation, accuracy and precision. The lower limit of quantitation was 0.02 ng/mL with a relative standard deviation of less than 10%. The present method provides an accurate, precise and sensitive tool for buspirone and was successfully applied to a pharmacokinetic study in eight subjects. Copyright © 2006 John Wiley & Sons, Ltd.