Identification and antimicrobial susceptibility of porcine bacteria that inhibit the growth of Brachyspira hyodysenteriae in vitro

• Published in: Journal of applied microbiology Vol. 108; no. 4; pp. 1271 - 1280
• Main Authors: Klose, V, Bruckbeck, R, Henikl, S, Schatzmayr, G, Loibner, A.P
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.04.2010; Blackwell Publishing Ltd; Blackwell
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; antibiotic susceptibility; Bacillus; Bacillus subtilis; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacteria - isolation & purification; bifidobacteria; Bifidobacterium thermophilum; Biological and medical sciences; Brachyspira hyodysenteriae; Brachyspira hyodysenteriae - classification; Brachyspira hyodysenteriae - genetics; Brachyspira hyodysenteriae - growth & development; Brachyspira hyodysenteriae - isolation & purification; DNA, Ribosomal; Enterococcus faecium; Fundamental and applied biological sciences. Psychology; Gastrointestinal Tract - microbiology; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - prevention & control; lactic acid bacteria; Lactobacillus; Lactobacillus amylovorus; Lactobacillus reuteri; Lactobacillus salivarius; Microbial Interactions - physiology; Microbial Sensitivity Tests; Microbiology; Phylogeny; porcine gastrointestinal tract; probiotic; Probiotics; Swine; Swine Diseases - microbiology; Swine Diseases - prevention & control; Lactic acid bacteria; bifidobacteria; Growth; Applied microbiology; Eubacteria; Bifidobacteriaceae; Identification; Bacillaceae; Antimicrobial agent; Brachyspira hyodysenteriae; Bacillales; Bacillus; Actinobacteria; Bacteria; Bifidobacteriales; Inhibition; antibiotic susceptibility; Digestive tract; Ungulata; Probiotic; porcine gastrointestinal tract; Bifidobacterium; In vitro; Pig; Vertebrata; Antibiotic; Mammalia; Sensitivity resistance; Actinobacteridae; Artiodactyla
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2009.04521.x

To identify bacilli, lactic acid bacteria and bifidobacteria that inhibit the growth of Brachyspira hyodysenteriae. A total of 80 isolates were obtained from various porcine intestinal compartments using selective conditions and grouped into 15 similarity clusters based on whole-cell protein profiles. Random amplified polymorphic DNA PCR patterns identified 24 genotypes. 16S rDNA sequencing assigned all genotypes, except eight aerobes, to established species (Bacillus subtilis, Enterococcus faecium, Lactobacillus salivarius, Lactobacillus mucosae, Lactobacillus reuteri, Lactobacillus amylovorus, Bifidobacterium thermophilum). According to their minimum inhibitory concentrations, four strains (Ent. faecium, Lact. reuteri, Lact. amylovorus, Bif. thermophilum) were susceptible to all clinically relevant antibiotics. Two lactobacilli showing multiresistance harboured the erm(B) determinant. A cross-section of eight representative strains was examined for growth suppression of two strains of Brach. hyodysenteriae, the aetiological agent of swine dysentery, and compared with intestinal strains derived from other animal sources. The Brachyspira strains were inhibited by strains of Lact. salivarius, Bif. thermophilum, Ent. faecium and B. subtilis. Three porcine strains of Ent. faecium, Bif. thermophilum and B. subtilis were found to be suitable as probiotic candidates because of their well-established identity, antibiotic susceptibility and antagonistic activity. For the first time, antagonistic activity of well-characterized porcine strains against Brach. hyodysenteriae is presented. These findings suggest that certain intestinal strains might have a potential as probiotic feed additives for prevention of swine dysentery.