Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high-speed counter-current chromatography
Alkannin and shikonin (A/S) and their derivatives have been found in the roots of several Boraginaceous species and are also produced through plant tissue cultures. The chiral compounds A/S are potent pharmaceutical substances with a wide spectrum of biological and pharmacological activities like wo...
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Published in | Biomedical chromatography Vol. 23; no. 2; pp. 182 - 198 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.02.2009
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Subjects | |
Online Access | Get full text |
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Summary: | Alkannin and shikonin (A/S) and their derivatives have been found in the roots of several Boraginaceous species and are also produced through plant tissue cultures. The chiral compounds A/S are potent pharmaceutical substances with a wide spectrum of biological and pharmacological activities like wound healing, antimicrobial, anti‐inflammatory, anticancer and antioxidant activity. High‐speed counter‐current chromatography (HSCCC) was applied for the first time to the separation, preparative isolation and purification of A/S and their esters from extracts of Alkanna tinctoria roots, as well as commercial samples. The constituents of HSCCC fractions and their purity were determined by high‐performance liquid chromatography–diode array detection–mass spectrometry (HPLC‐DAD‐MS), since DAD cannot detect oligomeric A/S derivatives that are present in most of the samples containing the respective monomeric derivatives. The purity of HSCCC fractions was compared with the one of fractions isolated by column chromatography (CC) using as stationary phases silica gel and Sephadex LH‐20. As shown, the purity of monomeric alkannin/shikonin was greater by HSCCC than CC separation of commercial A/S samples. Copyright © 2008 John Wiley & Sons, Ltd. |
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Bibliography: | This article was published online on 24 September 2008. An error was subsequently identified. This notice is included in the online and print versions indicating that both have been corrected on 3 November 2008. istex:4A3482E4A0C95A9C58AF1DB81B8E31C30962AAFC ArticleID:BMC1101 ark:/67375/WNG-1B64P36C-B ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0269-3879 1099-0801 |
DOI: | 10.1002/bmc.1101 |