PPR proteins – orchestrators of organelle RNA metabolism

• Published in: Physiologia plantarum Vol. 166; no. 1; pp. 451 - 459
• Main Authors: Rovira, Aleix Gorchs, Smith, Alison G.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.05.2019; Wiley Subscription Services, Inc
• Subjects: Arabidopsis Proteins - metabolism; Chlamydomonas - metabolism; Chloroplasts; Chloroplasts - metabolism; Coding; Dehydrogenase; Dehydrogenases; Electron transport chain; Genes; Metabolism; Mitochondria; NADH; NADH dehydrogenase; NADH Dehydrogenase - metabolism; Nicotinamide adenine dinucleotide; Plant Proteins - metabolism; Proteins; Regulators; Ribonucleic acid; RNA; RNA editing; RNA Editing - genetics; Splicing; Transcription
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/ppl.12950

Pentatricopeptide repeat (PPR) proteins are important RNA regulators in chloroplasts and mitochondria, aiding in RNA editing, maturation, stabilisation or intron splicing, and in transcription and translation of organellar genes. In this review, we summarise all PPR proteins documented so far in plants and the green alga Chlamydomonas. By further analysis of the known target RNAs from Arabidopsis thaliana PPR proteins, we find that all organellar‐encoded complexes are regulated by these proteins, although to differing extents. In particular, the orthologous complexes of NADH dehydrogenase (Complex I) in the mitochondria and NADH dehydrogenase‐like (NDH) complex in the chloroplast were the most regulated, with respectively 60 and 28% of all characterised A. thaliana PPR proteins targeting their genes.