Effects of body-color mutations on vitality: an attempt to establish easy-to-breed see-through medaka strains by outcrossing

• Published in: G3 : genes - genomes - genetics Vol. 3; no. 9; pp. 1577 - 1585
• Main Authors: Ohshima, Ayaka, Morimura, Noriko, Matsumoto, Chizuru, Hiraga, Ami, Komine, Ritsuko, Kimura, Tetsuaki, Naruse, Kiyoshi, Fukamachi, Shoji
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.09.2013
• Subjects: Alleles; Animals; Breeding; Crossing Over, Genetic; Genome; Genotype; Hybrid Vigor; Longevity; Membrane Transport Proteins - genetics; Mutant Screen Report; Mutation; Oryzias - genetics; Phenotype; Skin Pigmentation - genetics; oculocutaneous albinism type 2 (oca2); leucophore free; solute carrier family 45 member 2 (slc45a2); medaka; iridophoreless-1; guanineless
• ISSN: 2160-1836; 2160-1836
• DOI: 10.1534/g3.113.007575

"See-through" strains of medaka are unique tools for experiments: their skin is transparent, and their internal organs can be externally monitored throughout life. However, see-through fish are less vital than normally pigmented wild-type fish, which allows only skilled researchers to make the most of their advantages. Expecting that hybrid vigor (heterosis) would increase the vitality, we outcrossed two see-through strains (SK(2) and STIII) with a genetically distant wild-type strain (HNI). Fish with the see-through phenotypes were successfully restored in the F2 generation and maintained as closed colonies. We verified that genomes of these hybrid see-through strains actually consisted of approximately 50% HNI and approximately 50% SK(2) or STIII alleles, but we could not obtain evidence supporting improved survival of larvae or fecundity of adults, at least under our breeding conditions. We also found that four of the five see-through mutations (b(g8), i-3, gu, and il-1 but not lf) additively decrease viability. Given that heterosis could not overwhelm the viability-reducing effects of the see-through mutations, easy-to-breed see-through strains will only be established by other methods such as conditional gene targeting or screening of new body-color mutations that do not reduce viability.