Oligonucleotide dendrimers: Synthesis and use as polylabelled DNA probes
Oligonucleotide dendrimers were synthesized using a novel phosphoramidite synthon, tris-2,2,2-[3-(4,4′-dimethoxytrityloxy) propyloxymethyl]ethyl-N,N-diisopropylaminocyanethoxy phosphoramidite. Label, incorporated using [γ-32P]ATP and polynucleotide kinase, was increased in proportion to the number o...
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Published in | Nucleic acids research Vol. 25; no. 22; pp. 4447 - 4454 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
15.11.1997
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Subjects | |
Online Access | Get full text |
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Summary: | Oligonucleotide dendrimers were synthesized using a novel phosphoramidite synthon, tris-2,2,2-[3-(4,4′-dimethoxytrityloxy) propyloxymethyl]ethyl-N,N-diisopropylaminocyanethoxy phosphoramidite. Label, incorporated using [γ-32P]ATP and polynucleotide kinase, was increased in proportion to the number of 5′-ends. There was a similar increase in signal when these multiply labelled oligonucleotides were used as probes to oligonucleotide arrays. A dendrimeric oligonucleotide was used successfully as a primer in the PCR. The strand bearing the dendrimer was resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply-labelled, single-stranded probe. |
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Bibliography: | istex:C36A1E10D12AD6303E2AB14A23E20EBCC45958FA ark:/67375/HXZ-NNGQ4396-F ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0305-1048 1362-4962 1362-4962 |
DOI: | 10.1093/nar/25.22.4447 |