Insights into the xylan degradation system of Cellulomonas sp. B6: biochemical characterization of rCsXyn10A and rCsAbf62A

• Published in: Applied microbiology and biotechnology Vol. 106; no. 13-16; pp. 5035 - 5049
• Main Authors: Garrido, Mercedes María, Piccinni, Florencia Elizabeth, Landoni, Malena, Peña, María Jesús, Topalian, Juliana, Couto, Alicia, Wirth, Sonia Alejandra, Urbanowicz, Breeanna Rae, Campos, Eleonora
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2022; Springer; Springer Nature B.V
• Subjects: Arabinofuranosidase; Biodegradation; Biomedical and Life Sciences; Bioprocessing; Biorefineries; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Cellulose; Degradation; Dietary fiber; Enzymes; Gram-positive bacteria; Hemicellulose; L-Arabinofuranosidase; Life Sciences; Lignocellulose; Microbial Genetics and Genomics; Microbiological research; Microbiology; Oligosaccharides; Physiological aspects; Plant biomass; Polysaccharides; Proteomes; Residues; Saccharides; Substrates; Substrates (Biochemistry); Synergism; Wheat; Wheat bran; Xylan; Xylanase; Xylans; Argentina; GH10; Hemicellulose; Arabinofuranosidases; GH62; Xylanases
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-022-12061-3

Valorization of the hemicellulose fraction of plant biomass is crucial for the sustainability of lignocellulosic biorefineries. The Cellulomonas genus comprises Gram-positive Actinobacteria that degrade cellulose and other polysaccharides by secreting a complex array of enzymes. In this work, we studied the specificity and synergy of two enzymes, Cs Xyn10A and Cs Abf62A, which were identified as highly abundant in the extracellular proteome of Cellulomonas sp. B6 when grown on wheat bran. To explore their potential for bioprocessing, the recombinant enzymes were expressed and their activities were thoroughly characterized. r Cs Xyn10A is a GH10 endo-xylanase (EC 3.2.1.8), active across a broad pH range (5 to 9), at temperatures up to 55 °C. r Cs Abf62A is an α- l -arabinofuranosidase (ABF) (EC 3.2.1.55) that specifically removes α-1,2 and α-1,3- l -arabinosyl substituents from arabino-xylo-oligosaccharides (AXOS), xylan, and arabinan backbones, but it cannot act on double-substituted residues. It also has activity on p NPA. No differences were observed regarding activity when Cs Abf62A was expressed with its appended CBM13 module or only the catalytic domain. The amount of xylobiose released from either wheat arabinoxylan or arabino-xylo-oligosaccharides increased significantly when r Cs Xyn10A was supplemented with r Cs Abf62A, indicating that the removal of arabinosyl residues by r Cs Abf62A improved r Cs Xyn10A accessibility to β-1,4-xylose linkages, but no synergism was observed in the deconstruction of wheat bran. These results contribute to designing tailor-made, substrate-specific, enzymatic cocktails for xylan valorization. Key points • rCsAbf62A removes α-1,2 and α-1,3-L-arabinosyl substituents from arabino-xylo-oligosaccharides, xylan, and arabinan backbones. • The appended CBM13 of rCsAbf62A did not affect the specific activity of the enzyme. • Supplementation of rCsXyn10A with rCsAbf62A improves the degradation of AXOS and xylan.