Peptidomic analysis of human peripheral monocytes persistently infected by Chlamydia trachomatis

• Published in: Medical microbiology and immunology Vol. 196; no. 2; pp. 103 - 114
• Main Authors: KRAUSSE-OPATZ, Birgit, BUSMANN, Annette, KÖHLER, Lars, TAMMEN, Harald, MENZEL, Christoph, MÖHRING, Thomas, LE YONDRE, Nicolas, SCHMIDT, Cornelia, SCHULZ-KNAPPE, Peter, ZEIDLER, Henning, SELLE, Hartmut
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.06.2007; Springer Nature B.V
• Subjects: Bacteriology; Biological and medical sciences; Biology; Chlamydia; Chlamydia trachomatis; Chlamydia trachomatis - immunology; Chlamydia trachomatis - pathogenicity; Chlamydia trachomatis - physiology; Cytokines - immunology; Cytokines - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Immunology; Microbiology; Miscellaneous; Monocytes - immunology; Monocytes - metabolism; Monocytes - microbiology; Peptides; Peptides - immunology; Peptides - isolation & purification; Peptides - metabolism; Tandem Mass Spectrometry; Human; Infection; Monocytes; Immunology; Chlamydiaceae; Microbiology; Monocyte; Chlamydiales; Bacteria; Chlamydia trachomatis; Peptidomics
• ISSN: 0300-8584; 1432-1831
• DOI: 10.1007/s00430-006-0033-y

Peptidomic analysis using Differential Peptide Display (DPD) of human peripheral blood mononuclear cells (PBMC) mock-infected or persistently infected by Chlamydia trachomatis (CT) revealed 10 peptides, expressed upon CT infection. Analysis of these 10 candidates by tandem mass spectrometry enabled the determination of seven candidates as fragments from the precursors (I) ferritin heavy chain subunit, (II) HLA class II histocompatibility antigen, (III) vimentin, (IV) indoleamine 2,3-dioxygenase, (V and VI) pre-B cell enhancing factor (PBEF), and (VII) Interleukin-8 (CXCL8). The identified candidates proved the presence of anti-bacterial and immunologically active monocytic proteins after CT infection.