A New Robust Diagnostic Polymerase Chain Reaction for Determining the Mating Status of Female Anopheles gambiae Mosquitoes

The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mat...

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Bibliographic Details
Published inThe American journal of tropical medicine and hygiene Vol. 77; no. 3; pp. 485 - 487
Main Authors Ng'habi, Kija R, Horton, Ashley, Knols, Bart G. J, Lanzaro, Gregory C
Format Journal Article
LanguageEnglish
Published Lawrence, KS ASTMH 01.09.2007
Allen Press
Subjects
Animals
Anopheles - physiology
Biological and medical sciences
degeneration
drosophila-melanogaster
evolution
Female
genome
Human protozoal diseases
Infectious diseases
Malaria
Male
Medical sciences
miranda
neo-y
Parasitic diseases
Polymerase Chain Reaction - methods
Protozoal diseases
Reproduction
sequences
sex
Sexual Behavior, Animal - physiology
Y Chromosome - chemistry
y-chromosome
Protozoal disease
Malaria
Insecta
Parasitosis
Infection
Mating
Polymerase chain reaction
Anopheles gambiae
Arthropoda
DNA
Culicidae
Female
Tropical medicine
Diagnosis
Molecular biology
Invertebrata
Vector
Diptera
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Summary:The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.
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ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2007.77.485