Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-κB pathway in human dermal fibroblasts

• Published in: Experimental & molecular medicine Vol. 41; no. 8; pp. 548 - 554
• Main Authors: Lee, Young-Rae, Noh, Eun-Mi, Jeong, Eun-Yong, Yun, Seok-Kweon, Jeong, Young-Ju, Kim, Jong-Hyeon, Kwon, Kang-Beom, Kim, Byeong-Soo, Lee, Sung-Ho, Park, Chang-Sik, Kim, Jong-Suk
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 31.08.2009; Korean Society of Medical Biochemistry and Molecular Biology; 생화학분자생물학회
• Subjects: Biomedical and Life Sciences; Biomedicine; Medical Biochemistry; Molecular Medicine; Original; Stem Cells; 생화학; NF-κB; ultraviolet rays; skin aging; cordycepin; matrix metalloproteinases
• ISSN: 1226-3613; 2092-6413
• DOI: 10.3858/emm.2009.41.8.060

Cordycepin (3'-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-κB activity, which was determined by IκBα degradation, nuclear localization of p50 and p65 subunit, and NF-κB binding activity. However, UVB-induced NF-κB activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-κB activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.