Immunohistochemical studies of steroidogenic enzymes (aromatase, 17α-hydroxylase and cholesterol side-chain cleavage cytochromes P-450) in sex cord-stromal tumors of the ovary

• Published in: Human pathology Vol. 20; no. 5; pp. 452 - 457
• Main Authors: Sasano, Hironobu, Okamoto, Mitsuhiro, Mason, J.Ian, Simpson, Evan R., Mendelson, Carrol R., Sasano, Nobuaki, Silverberg, Steven G.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.05.1989; Elsevier
• Subjects: Aromatase - metabolism; Biological and medical sciences; Cholesterol Side-Chain Cleavage Enzyme - metabolism; cytochromes P-450; Female; Female genital diseases; Fibroma - enzymology; Granulosa Cell Tumor - enzymology; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; Leydig Cell Tumor - enzymology; Medical sciences; ovarian neoplasms; Ovarian Neoplasms - enzymology; Sertoli Cell Tumor - enzymology; Steroid 17-alpha-Hydroxylase - metabolism; Steroid Hydroxylases - metabolism; steroidogenesis; Thecoma - enzymology; Tumors; steroidogenesis; ovarian neoplasms; cytochromes P-450; Human; Secretory tumor; Pathology; Ovary; Enzyme; Immunocytochemistry; Female; Tumor; Steroidogenesis; Malignant tumor; Female genital diseases
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/0046-8177(89)90010-5

Aromatase, 17α-hydroxylase, and cholesterol side-chain cleavage P-450 cytochromes (P-450 arom , P-450 17α, and P-450 scc , respectively) were immunohistochemically localized in nine granulosa cell tumors, 15 thecomas, ten Sertoli-Leydig cell tumors, two steroid cell tumors, five fibromas, and five sclerosing stromal tumors. In the thecomas, P-450 scc and P-450 17α were positive in luteinized theca cells and in cells with vacuolated cytoplasm, while P-450 arom was not observed. In the steroid cell tumors, all the P-450 cytochromes were intensely stained. P-450 scc and P-450 17α were present in cells with vacuolated cytoplasm in two cases of sclerosing stromal tumor. P-450 arom was weakly demonstrated in one of the granulosa cell tumors. P-450 17α, P-450 scc , and P-450 arom were all faintly stained in the Sertoli-Leydig cell tumors. No P-450 cytochrome immunoreactivity was observed in any fibroma.