Mono- and digalactosyldiacylglycerol composition of dinoflagellates. III. Four cold-adapted, peridinin-containing taxa and the presence of trigalactosyldiacylglycerol as an additional glycolipid

• Published in: European journal of phycology Vol. 44; no. 3; pp. 439 - 445
• Main Authors: Gray, Cynthia G., Lasiter, Andrew D., Leblond, Jeffrey D.
• Format: Journal Article
• Language: English
• Published: Taylor & Francis Group 01.08.2009
• Subjects: algae; cold-adapted; DGDG; dinoflagellate; Dinoflagellates; fatty acid; Glycolipids; Gymnodinium; lipid; MGDG; peridinin; Peridinium; Scrippsiella; TGDG; Woloszynskia
• ISSN: 0967-0262; 1469-4433
• DOI: 10.1080/09670260902787977

Despite their importance in marine and freshwater microalgal assemblages, cold-adapted dinoflagellates have been the subject of few comprehensive lipid studies, particularly with respect to those lipids that comprise plastid membranes. In an effort to understand the differences between warm- and cold-adapted dinoflagellate glycolipid composition, four peridinin-containing, cold-adapted dinoflagellates were surveyed for intact forms of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), two common plastid lipids, using positive-ion electrospray ionization/mass spectrometry (ESI/MS) and electrospray ionization/mass spectrometry/mass spectrometry (ESI/MS/MS). It was determined that the dominant forms of MGDG and DGDG in these cold-adapted, peridinin-containing dinoflagellates possessed C 18 fatty acids and did not, with the exception of a 20:5/18:5 form of DGDG in a cold-adapted Gymnodinium sp. from the Baltic Sea, have C 20 fatty acids. This finding is in contrast to an earlier study of 35 peridinin-containing, warm-adapted dinoflagellates, which discovered a cluster dominated by C 18 fatty acids and a cluster dominated by both C 20 and C 18 fatty acids. The key difference in MGDG and DGDG production between the former group and the cold-adapted dinoflagellates examined in this study is that the cold-adapted species' DGDG fatty acids were less saturated. Each cold-adapted dinoflagellate possessed both 18:5/18:5 and 18:5/18:4 DGDG, while most of the warm-adapted dinoflagellates contained only 18:5/18:4 DGDG. This survey also revealed the presence of a putative 18:1/14:0 trigalactosyldiacylglycerol (TGDG) as a dominant glycolipid in Gymnodinium sp. TGDG, previously unreported in dinoflagellates, was also discovered in Gymnodinium sp. in the forms of 18:1/16:0 and 18:1/18:1 TGDG, as minor lipids. Since the fatty acids associated with TGDG are not those found with dominant forms of MGDG or DGDG, TGDG may be produced by a different biosynthetic pathway.