Potent Antisense Oligonucleotides to the Human Multidrug Resistance-1 mRNA Are Rationally Selected by Mapping RNA-Accessible Sites With Oligonucleotide Libraries

• Published in: Nucleic acids research Vol. 24; no. 10; pp. 1901 - 1907
• Main Authors: Peng Ho, Siew, Britton, Dustin H. O., Stone, Barry A., Behrens, Davette L., Leffet, Lynn M., Hobbs, Frank W., Miller, Jeff A., Trainor, George L.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 15.05.1996
• Subjects: ATP Binding Cassette Transporter, Subfamily B, Member 1 - antagonists & inhibitors; ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics; ATP Binding Cassette Transporter, Subfamily B, Member 1 - physiology; Base Sequence; Gene Library; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotides, Antisense - analysis; Oligonucleotides, Antisense - pharmacology; Rhodamines - metabolism; Ribonuclease H - metabolism; RNA, Messenger - analysis; RNA, Messenger - chemistry; Tumor Cells, Cultured
• ISSN: 0305-1048; 1362-4962; 1362-4962
• DOI: 10.1093/nar/24.10.1901

Antisense oligonucleotides can vary significantly and unpredictably in their ability to inhibit protein synthesis. Libraries of chimeric oligonucleotides and RNase H were used to cleave and thereby locate sites on human multidrug resistance-1 RNA transcripts that are relatively accessible to oligonucleotide hybridization. In cell culture, antisense sequences designed to target these sites were significantly more active than oligonucleotides selected at random. This methodology should be generally useful for identification of potent antisense sequences. Correlation between oligonucleotide activity in the cell culture assay and in an in vitro RNase H assay supports the proposed role of the enzyme in the mechanism of antisense suppression in the cell.