Stress-Induced Reorganization of the Mycobacterial Membrane Domain

• Published in: mBio Vol. 9; no. 1
• Main Authors: Hayashi, Jennifer M, Richardson, Kirill, Melzer, Emily S, Sandler, Steven J, Aldridge, Bree B, Siegrist, M Sloan, Morita, Yasu S
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 23.01.2018
• Subjects: Cell Division; cell envelope; Cell Fractionation; Cell Membrane - metabolism; Centrifugation, Density Gradient; membrane proteins; membranes; Mycobacterium; Mycobacterium tuberculosis - growth & development; peptidoglycan; Peptidoglycan - metabolism; stress response; Stress, Physiological; membranes; Mycobacterium; peptidoglycan; membrane proteins; stress response; cell envelope
• ISSN: 2161-2129; 2150-7511
• DOI: 10.1128/mBio.01823-17

Cell elongation occurs primarily at the mycobacterial cell poles, but the molecular mechanisms governing this spatial regulation remain elusive. We recently reported the presence of an intracellular membrane domain (IMD) that was spatially segregated from the conventional plasma membrane in The IMD is enriched in the polar region of actively elongating cells and houses many essential enzymes involved in envelope biosynthesis, suggesting its role in spatially restricted elongation at the cell poles. Here, we examined reorganization of the IMD when the cells are no longer elongating. To monitor the IMD, we used a previously established reporter strain expressing fluorescent IMD markers and grew it to the stationary growth phase or exposed the cells to nutrient starvation. In both cases, the IMD was delocalized from the cell pole and distributed along the sidewall. Importantly, the IMD could still be isolated biochemically by density gradient fractionation, indicating its maintenance as a membrane domain. Chemical and genetic inhibition of peptidoglycan biosynthesis led to the delocalization of the IMD, suggesting the suppression of peptidoglycan biosynthesis as a trigger of spatial IMD rearrangement. Starved cells with a delocalized IMD can resume growth upon nutrient repletion, and polar enrichment of the IMD coincides with the initiation of cell elongation. These data reveal that the IMD is a membrane domain with the unprecedented capability of subcellular repositioning in response to the physiological conditions of the mycobacterial cell. Mycobacteria include medically important species, such as the human tuberculosis pathogen The highly impermeable cell envelope is a hallmark of these microbes, and its biosynthesis is a proven chemotherapeutic target. Despite the accumulating knowledge regarding the biosynthesis of individual envelope components, the regulatory mechanisms behind the coordinated synthesis of the complex cell envelope remain elusive. We previously reported the presence of a metabolically active membrane domain enriched in the elongating poles of actively growing mycobacteria. However, the spatiotemporal dynamics of the membrane domain in response to stress have not been examined. Here, we show that the membrane domain is spatially reorganized when growth is inhibited in the stationary growth phase, under nutrient starvation, or in response to perturbation of peptidoglycan biosynthesis. Our results suggest that mycobacteria have a mechanism to spatiotemporally coordinate the membrane domain in response to metabolic needs under different growth conditions.