Lack of activation of peripheral blood dendritic cells in human pregnancies complicated by intrauterine growth restriction

• Published in: Placenta (Eastbourne) Vol. 34; no. 1; pp. 35 - 41
• Main Authors: Cappelletti, M, Giannelli, S, Martinelli, A, Cetin, I, Colombo, E, Calcaterra, F, Mavilio, D, Della Bella, S
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.01.2013; Elsevier
• Subjects: Adult; Biological and medical sciences; Blood Cell Count; Blood Cells - immunology; Blood Cells - pathology; Case-Control Studies; Costimulatory molecules; Cytokines; Cytokines - blood; Dendritic Cells - immunology; Dendritic Cells - pathology; Down-Regulation - immunology; Embryology: invertebrates and vertebrates. Teratology; Female; Fetal Growth Retardation - blood; Fetal Growth Retardation - immunology; Fetal Growth Retardation - pathology; Fundamental and applied biological sciences. Psychology; Humans; Infant, Newborn; Inflammation Mediators - blood; Internal Medicine; Intrauterine growth restriction; Male; Obstetrics and Gynecology; Peripheral blood dendritic cells; Pregnancy; Intrauterine growth restriction; Costimulatory molecules; Cytokines; Peripheral blood dendritic cells; Pregnancy; Human; Dendritic cell; Fetal diseases; Vertebrata; Blood cell; Intrauterine growth retardation; Mammalia; Pregnancy disorders; Complication; Activation
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/j.placenta.2012.10.016

Abstract Introduction The state of activation of dendritic cells (DCs) at the feto-maternal interface critically contributes to optimal decidual immune responses needed to support fetal-placental development. We recently demonstrated that during healthy pregnancy also peripheral blood DCs (PBDCs), which are easily accessible, are activated as well. In this study, to investigate a possible involvement of DCs in intrauterine growth restriction (IUGR), we evaluated whether PBDCs in pregnancy complicated by IUGR may be altered compared with PBDCs in healthy pregnancy. Methods PBDCs from 12 pregnant women with primary IUGR, 21 healthy pregnant and 19 nonpregnant women were analyzed by flow cytometric analysis of whole-blood samples collected at a single time point. Results The number of plasmacytoid PBDCs was significantly reduced in women with IUGR pregnancy. Myeloid and plasmacytoid PBDCs in IUGR lacked the state of activation (assessed as CD80, CD86, CD40 expression) and the shift to a proinflammatory pattern of cytokine production occurring during healthy pregnancy. Discussion To our knowledge, this is the first study investigating the state of PBDC activation in IUGR pregnancy. Our results are in accordance with a previous study reporting a lower expression of activation and maturation markers by decidual DCs in IUGR placentas. Conclusions The reduced activation of PBDCs in IUGR pregnancy may possibly reflect a reduced activation of decidual DCs. If confirmed at the feto-maternal interface, the alterations of DCs described in IUGR pregnancy have the potential to negatively impact on vascular development during gestation. These observations may therefore broaden our understanding of IUGR pathogenesis.