Microbial asymmetric oxidation of 2-butyl-1,3-propanediol

Microbial asymmetric oxidation of 2-butyl-1,3-propanediol was investigated for an efficient synthesis of S- and R-enantiomers of 2-hydroxymethylhexanoic acid (2-HMHA). From an intensive survey of the stocked bacterial strains, Acetobacter pasteurianus IAM 12073 and Pseudomonas putida IFO 3738 were f...

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Published inApplied microbiology and biotechnology Vol. 76; no. 1; pp. 61 - 65
Main Authors MITSUKURA, K, UNO, T, YOSHIDA, T, NAGASAWA, T
Format Journal Article
LanguageEnglish
Published Berlin Springer 01.08.2007
Springer Nature B.V
Subjects
Acetobacter - metabolism
Acetobacter pasteurianus
Acids
Biological and medical sciences
Biotechnology
Biotransformation
Fundamental and applied biological sciences. Psychology
Microbiology
Oxidation
Oxidation-Reduction
Propylene Glycol - metabolism
Pseudomonas putida - metabolism
Studies
Propanediol
Oxidation
Microorganism
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Summary:Microbial asymmetric oxidation of 2-butyl-1,3-propanediol was investigated for an efficient synthesis of S- and R-enantiomers of 2-hydroxymethylhexanoic acid (2-HMHA). From an intensive survey of the stocked bacterial strains, Acetobacter pasteurianus IAM 12073 and Pseudomonas putida IFO 3738 were found to show the highest S- and R-2-HMHA-producing activity, respectively. Under optimized conditions, A. pasteurianus (351 mg dry cell weight) and P. putida (642 mg dry cell weight) cells produced 12.0 g l(-1) S-2-HMHA with 89% enantiomeric excess (e.e.) at 24 h of incubation and 5.1 g l(-1) R-2-HMHA with 94% e.e. at 35 h of incubation from 2-butyl-1,3-propanediol.
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ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-007-0974-0