Expression and developmental regulation of the NMDA receptor subunits in the kidney and cardiovascular system
Department of Pediatrics, Divisions of 1 Neonatology, 2 Nephrology, and 4 Cardiology and 5 Departments of Pediatrics and Microbiology and the Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112; and 3 Department of Medicine, 6 Veteran...
Saved in:
Published in | American journal of physiology. Regulatory, integrative and comparative physiology Vol. 283; no. 4; pp. 964 - R971 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.10.2002
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Department of Pediatrics, Divisions of
1 Neonatology, 2 Nephrology, and
4 Cardiology and 5 Departments of
Pediatrics and Microbiology and the Stanley S. Scott Cancer Center,
Louisiana State University Health Sciences Center, New Orleans,
Louisiana 70112; and 3 Department of Medicine,
6 Veterans Administration Medical Center,
University of Florida, Gainesville, Florida 32611
Antagonists to the
N -methyl- D -aspartate (NMDA) receptor bind to
various extraneuronal tissues. We therefore assessed the expression of
the main NMDA subunit, NR1, in various tissues. We demonstrate that NR1
appears to be most abundant in the rat kidney and heart. NR1 is present
in total rat kidney, cortex, and medulla. Of the NR2 subunits, only the
NR2C subunit protein is present in the kidney. The abundance of the NR1
subunit protein increases with kidney development. Both NR1 and NR2C
are present in opossum kidney, Madin-Darby canine kidney, and
LLC-PK 1 cells. Immunohistochemistry studies show that the
NR1 subunit is present in the renal proximal tubule. NR1 is abundant in
the atrium and ventricle but is also expressed in the aorta and
pulmonary artery. The NR2 subunits are not expressed in the heart. NR1
subunit protein expression is constant throughout heart development.
Finally, the NR1 subunit protein is expressed in heart cells (H9c2)
grown in culture. These studies reveal the presence of the NMDA
receptor in the kidney and the cardiovascular system.
N -methyl- D -aspartate receptor; development |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0363-6119 1522-1490 |
DOI: | 10.1152/ajpregu.00629.2001 |