BMP-2 enhances the migration and proliferation of hypoxia-induced VSMCs via actin cytoskeleton, CD44 and matrix metalloproteinase linkage

• Published in: Experimental cell research Vol. 368; no. 2; pp. 248 - 257
• Main Authors: Yang, Min, Fan, Zhiqin, Wang, Fei, Tian, Zhi-hua, Ma, Bo, Dong, Bin, Li, Zhongwu, Zhang, Ming, Zhao, Wei
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.07.2018
• Subjects: 60 APPLIED LIFE SCIENCES; ACTIN; Actin cytoskeleton; ARTERIOSCLEROSIS; BMP-2; CD44; FLUORESCENCE; Hypoxia; Matrix metalloproteinase; MEMBRANE PROTEINS; MICROTUBULES; CCA; PEX; ECA; ICA; MMPs; Matrix metalloproteinase; BMP-2; VMSCs; IP3; ECM; Hypoxia; CABG; Actin cytoskeleton; EC; CD44
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/j.yexcr.2018.05.004

The persistent proliferation of hypoxia-induced vascular smooth muscle cells (VSMCs) in the arterial wall underlie the development of atherosclerosis. However, the mechanism that regulates the behavior of VSMCs, which involve in actin aggregation, and impedes their migration is still elusive. Here, we report that bone morphogenetic protein 2 (BMP-2) leads to enrichment of CD44 and F-actin stress fiber and secretion of matrix metalloproteinases-2 (MMP-2) during hypoxia in vitro and following artificial hypoxia-induced atherosclerosis exacerbation in vivo. To test this hypothesis, fluorescence immunostaining, immune-hybridization and flow cytometry analyses were performed to understand the relationship among BMP-2, CD44 and MMP-2 linkage. The cellular actin cytoskeleton was reduced, and smaller adhesion plaques were formed in hypoxia-induced T/G HA-VSMC cell line, but BMP-2 against disruption of F-actin and increase the motility and migration behaviors of VSMC during hypoxic cultured. Aggregation of F-actin dependents on the interaction between the cell surface integral membrane protein CD44 and Vinculin which enhanced by rBMP-2. This activity of Actin/CD44/ linkage was inhibited by competing with the active site of the CD44 using recombined the hemopexin-like C-terminal domain (PEX) of MMP-2. These results lead to the proliferation and migration of VSMCs were inhibited in response to MMP-2 activity when the cell is in a hypoxic environment. Collectively, our discovery indicates that BMP-2 could enhance migration and proliferation of hypoxia-induced VSMCs via the Actin/CD44/MMP-2 molecular pathway. •BMP-2 upregulates hypoxia-induced VSMC migration.•BMP-2 reduces F-actin disaggregate.•BMP-2 increase CD44 production.•PEX inhibits MMP-2 and CD44 combining.