An assessment of the genomics, comparative genomics and cellulose degradation potential of Mucilaginibacter polytrichastri strain RG4-7

• Published in: Bioresource technology Vol. 297; p. 122389
• Main Authors: Wang, Zi Yue, Wang, Rui Xue, Zhou, Jun Shi, Cheng, Jian Fei, Li, Yan Hong
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2020
• Subjects: Bacteroidetes; Carbohydrate Metabolism; Cellulase; Cellulose; Cellulose degradation; Comparative genomic analysis; Genomic feature; Genomics; Mucilaginibacter; Whole Genome Sequencing; Genomic feature; Comparative genomic analysis; Mucilaginibacter; Cellulose degradation
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2019.122389

[Display omitted] •Mucilaginibacter polytrichastri RG4-7 had the cellulose degradation capability.•High CMCase and betaglucosidase activity could be detected in RG4-7.•The cellulase related genes in RG4-7 were predicted based on its genome sequence.•The GH family determines cellulose degradation in RG4-7 might be GH9, GH1 and GH3. In this study, whole genome sequencing and comparative genomic analyses were performed for Mucilaginibacter polytrichastri RG4-7 and its carboxymethyl cellulose degradation potential was assessed. The results showed that the genome of strain RG4-7 was 5.84 Mb and contained 5019 predicted genes, in which a high proportion of strain-specific genes were related to carbohydrate metabolism. The carboxymethyl cellulose (CMC) degradation and cellulase activity tests revealed the strong cellulose degradation ability, CMCase and β-glucosidase activity in strain RG4-7. Real-time RT-PCR testing of most cellulose degradation related glycoside hydrolase (GH) families showed that GH9 (OKS85969), GH1 (OKS85832), GH3 (OKS89331 and OKS85615) were significantly up-regulated when strain RG4-7 was inoculated with CMC-Na, which suggested that GH9, GH1 and GH3 might determine its cellulose degradation ability. Certainly, further research need to be done to elucidate cellulose degradation mechanisms in strain RG4-7 in order to develop its industrial application value in lignocellulosic biomass degradation and waste management.